LOCUS       sequence-87739-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA tagged human MEK1 C121S from blasticidin resistance 
            retroviral vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pWZLblasti-HA-MEK1 C121S
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             69..95
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atcctccctt tatccagccc tcactccttc tctaggcgcc ggccggatcc gatatcgccg
       61 ccaccatgta cccatacgat gttccagatt acgctcccaa gaagaagccg acgcccatcc
      121 agctgaaccc ggcccccgac ggctctgcag ttaacgggac cagctctgcg gagaccaact
      181 tggaggcctt gcagaagaag ctggaggagc tagagcttga tgagcagcag cgaaagcgcc
      241 ttgaggcctt tcttacccag aagcagaagg tgggagaact gaaggatgac gactttgaga
      301 agatcagtga gctgggggct ggcaatggcg gtgtggtgtt caaggtctcc cacaagcctt
      361 ctggcctggt catggccaga aagctaattc atctggagat caaacccgca atccggaacc
      421 agatcataag ggagctgcag gttctgcatg agtccaactc tccgtacatc gtgggcttct
      481 atggtgcgtt ctacagcgat ggcgagatca gtatctgcat ggagcacatg gatggaggtt
      541 ctctggatca agtcctgaag aaagctggaa gaattcctga acaaatttta ggaaaagtta
      601 gcattgctgt aataaaaggc ctgacatatc tgagggagaa gcacaagatc atgcacagag
      661 atgtcaagcc ctccaacatc ccagtcaact cccgtgggga gatcaagctc tgtgactttg
      721 gggtcagcgg gcagctcatc gactccatgg ccaactcctt cgtgggcaca aggtcctaca
      781 tgtcgccaga aagactccag gggactcatt actctgtgca gtcagacatc tggagcatgg
      841 gactgtctct ggtagagatg gcggttggga ggtatcccat ccctcctcca gatgccaagg
      901 agctggagct gatgtttggg tgccaggtgg aaggagatgc ggctgaga
//
