LOCUS       sequence-87751-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIc F 4-5
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    156..280
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(317..322,323..340,341..347)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(467..486)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 acgactcact atagggcgaa ttggcggaag gccgtcaagg ccacgtgtct tgtccaggcg
       61 cgccatgagc tctaaagctt cgctcgagct gcggccgcac ggatcctcga attcccaagc
      121 ttatctcgac ccgaagacaa tgtcgcggtg agaccacaag tttgtacaaa aaagctgaac
      181 gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga
      241 ctacataata ctgtaaaaca caacatatcc agtcactatg gcggcccctc gaggggccgc
      301 attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag
      361 ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
      421 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
      481 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
      541 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
      601 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
      661 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
      721 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
      781 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctca
//
