LOCUS       sequence-87754-e         600 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIIalpha F A-B
FEATURES             Location/Qualifiers
     source          1..600
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(83..388)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(185..204)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 actgatagtt taaaccagag gtctcacaga cagaaagtct tcgtcgacct gcagactggc
       61 tgtgtataag ggagcctgac atttatattc cccagaacat caggttaatg gcgtttttga
      121 tgtcattttc gcggtggctg agatcagcca cttcttcccc gataacggaa accggcacac
      181 tggccatatc ggtggtcatc atgcgccagc tttcatcccc gatatgcacc accgggtaaa
      241 gttcacgggg gactttatct gacagcagac gtgcactggc cagggggatc accatccgtc
      301 gcccgggcgt gtcaataata tcactctgta catccacaaa cagacgataa cggctctctc
      361 ttttataggt gtaaacctta aactgcattt caccagcccc tgttctcgtc agcaaaagag
      421 ccgttcattt caataaaccg ggcgacctca gccatccctt cctgattttc cgctttccag
      481 cgttcggcac gcagacgacg ggcttcattc tgcatggttg tgcttaccag accggagata
      541 ttgacatcat atatgccttg agcaactgat agctgtcgct gtcaactgtc actgtaatac
//
