LOCUS       sequence-87755-e         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIIbeta F A-B
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(98..403)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(200..219)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tatatcctgt caaacactga tagtttaaac cagaggtctc acagacagaa agtcttcgtc
       61 gacctgcaga ctggctgtgt ataagggagc ctgacattta tattccccag aacatcaggt
      121 taatggcgtt tttgatgtca ttttcgcggt ggctgagatc agccacttct tccccgataa
      181 cggaaaccgg cacactggcc atatcggtgg tcatcatgcg ccagctttca tccccgatat
      241 gcaccaccgg gtaaagttca cgggggactt tatctgacag cagacgtgca ctggccaggg
      301 ggatcaccat ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc acaaacagac
      361 gataacggct ctctctttta taggtgtaaa ccttaaactg catttcacca gcccctgttc
      421 tcgtcagcaa aagagccgtt catttcaata aaccgggcga cctcagccat cccttcctga
      481 ttttccgctt tccagcgttc ggcacgcaga cgacgggctt cattctgcat ggttgtgctt
      541 accagaccgg agatattgac atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa
      601 ctgtcactgt aatacgctgc ttcatagcat acctcttttt gacatacttc gggtatacat
      661 atcagtatat attcttatac cgcaaaaatc a
//
