LOCUS       sequence-87758-e        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI A-B pNOS   c1
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        111..290
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        143..326
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     complement(414..431)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(414..430)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(423..445)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(639..658)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     758..780
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(818..836)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agcttgcatg caggacggtc tcagcgggat catgagcgga gaattaaggg agtcacgtta
       61 tgacccccgc cgatgacgcg ggacaagccg ttttacgttt ggaactgaca gaaccgcaac
      121 gttgaaggag ccactcagcc gcgggtttct ggagtttaat gagctaagca catacgtcag
      181 aaaccattat tgcgcgttca aaagtcgcct aaggtcacta tcagctagca aatatttctt
      241 gtcaaaaatg ctccactgac gttccataaa ttcccctcgg tatccaatta gagtctcata
      301 ttcactctca atccaaataa tctgcaccgg atcttctgag agacctacct ctgcagtcga
      361 cgggcccggg atccgatatc tagatgcatt cgcgaggtac cgagctcgaa ttcactggcc
      421 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
      481 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
      541 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
      601 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
      661 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
      721 ctcccggcat ccgcttacag acaagctgtg accttctccg ggagctgcat gtgtcagagg
      781 ttttcaccgt catcaccgaa acgcgcgaga caaaagggcc tcgtgatacg cctattttta
      841 taggttaatg tcatgagcgg cgttgtgaca atttaccgaa caactccgcg gccgggaagc
      901 cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca aagtgcatca
      961 cttcttcccg tatgcccaac tttgtataga gagccactgc gggatcgtca ccgtaatctg
//
