LOCUS       sequence-87759-e         616 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIIbeta fin
FEATURES             Location/Qualifiers
     source          1..616
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..355)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(152..171)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aaagtcttcg tcgacctgca gactggctgt gtataaggga gcctgacatt tatattcccc
       61 agaacatcag gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt
      121 cttccccgat aacggaaacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt
      181 catccccgat atgcaccacc gggtaaagtt cacgggggac tttatctgac agcagacgtg
      241 cactggccag ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat
      301 ccacaaacag acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac
      361 cagcccctgt tctcgtcagc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc
      421 atcccttcct gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc
      481 atggttgtgc ttaccagacc ggagatattg acatcatata tgccttgagc aactgatagc
      541 tgtcgctgtc aactgtcact gtaatacgct gcttcatagc atacctcttt ttgacatact
      601 tcgggtatac atatca
//
