LOCUS       sequence-87764-e         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIbeta F 5-6
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(103..408)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(205..224)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gccaatatat cctgtcaaac actgatagtt taaaccagag aagacaacag agacatgaga
       61 ccgtcgacct gcagactggc tgtgtataag ggagcctgac atttatattc cccagaacat
      121 caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca cttcttcccc
      181 gataacggaa accggcacac tggccatatc ggtggtcatc atgcgccagc tttcatcccc
      241 gatatgcacc accgggtaaa gttcacgggg gactttatct gacagcagac gtgcactggc
      301 cagggggatc accatccgtc gcccgggcgt gtcaataata tcactctgta catccacaaa
      361 cagacgataa cggctctctc ttttataggt gtaaacctta aactgcattt caccagcccc
      421 tgttctcgtc agcaaaagag ccgttcattt caataaaccg ggcgacctca gccatccctt
      481 cctgattttc cgctttccag cgttcggcac gcagacgacg ggcttcattc tgcatggttg
      541 tgcttaccag accggagata ttgacatcat atatgccttg agcaactgat agctgtcgct
      601 gtcaactgtc actgtaatac gctgcttcat agcatacctc tttttgacat acttcgggta
      661 tacatatcag tatatattct tataccgcaa aaatcagcgc gcaaatacgc atactgttat
      721 ctggctttta gtaagccgga tcctctagat tacgcccc
//
