LOCUS       sequence-87769-e         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIbeta R 2-3
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(47..352)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(149..168)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gagaccgtcg acctgcagac tggctgtgta taagggagcc tgacatttat attccccaga
       61 acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctgagatca gccacttctt
      121 ccccgataac ggaaaccggc acactggcca tatcggtggt catcatgcgc cagctttcat
      181 ccccgatatg caccaccggg taaagttcac gggggacttt atctgacagc agacgtgcac
      241 tggccagggg gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc tgtacatcca
      301 caaacagacg ataacggctc tctcttttat aggtgtaaac cttaaactgc atttcaccag
      361 cccctgttct cgtcagcaaa agagccgttc atttcaataa accgggcgac ctcagccatc
      421 ccttcctgat tttccgcttt ccagcgttcg gcacgcagac gacgggcttc attctgcatg
      481 gttgtgctta ccagaccgga gatattgaca tcatatatgc cttgagcaac tgatagctgt
      541 cgctgtcaac tgtcactgta atacgctgct tcatagcata cctctttttg acatacttcg
      601 ggtatacata tcagtatata ttcttatacc gcaaaaatca gcgcgcaaat acgcatactg
      661 ttatctggct tttagtaagc cggatcctct agattac
//
