LOCUS       sequence-87772-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Csy4 and Cas9 D10A nickase expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Tsai SQ, Wyvekens N, Khayter C, Foden JA, Thapar V, Reyon D, Goodwin
            MJ, Aryee MJ, Joung JK
  TITLE     Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome 
            editing.
  JOURNAL   Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908.
  PUBMED    24770325
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908."
COMMENT     Sequence Label: pNW3
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(64..129)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(136..156)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 attggccaca ccagccacca ccttctgata ggcagcctgc acctgaggag tgcggccgct
       61 tcacttgtca tcgtcatcct tgtaatcgat gtcatgatct ttataatcac cgtcatggtc
      121 tttgtagtcg ctcgagactt tcctcttctt cttgggggat ccgtcacccc caagctgtga
      181 caaatctatc cgagtttcat ataatcccgt gatggattgg tgaatcagtg tcgcgtctag
      241 cacctccttg gtagaagtgt atcgtttgcg atctatcgtt gtgtcaaaat acttgaatgc
      301 ggctggagcg ccgaggttgg taagagtaaa caaatggata atattttccg cctgctcacg
      361 tatgggttta tccctgtgct tgttgtatgc gcttaatact ttgtccagat tggcatcagc
      421 taggatgact ctcttactga attccgaaat ttgctctatg atttcgtcga gataatgttt
      481 gtgctgctca acaaaaagtt gcttctgttc gttatcttca ggtgaacctt tcaacttctc
      541 gtaatgggac gctaaataca ggaaattcac gtatttagac ggtagtgcga gttcgttccc
      601 cttttgaagc tctccggcgc tagccaacat ccgttttcgg ccattttcta actcaaacag
      661 actatacttt ggtagtttaa ttatgagatc cttttttact tccttgtaac ctttcgcctc
      721 aaggaagtcg atggggttct tttcaaaaga cgagcgctcc ataatcgtta tccccaa
//
