LOCUS       sequence-87774-e         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Csy4 and Cas9 D10A nickase expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Tsai SQ, Wyvekens N, Khayter C, Foden JA, Thapar V, Reyon D, Goodwin
            MJ, Aryee MJ, Joung JK
  TITLE     Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome 
            editing.
  JOURNAL   Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908.
  PUBMED    24770325
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908."
COMMENT     Sequence Label: pNW3
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aacgactcag aaggggcaaa aaaacagtcg agagcggatg aagagaatag aagagggtat
       61 taaagaactg ggcagccaga tcttaaagga gcatcctgtg gaaaataccc aattgcagaa
      121 cgagaaactt tacctctatt acctacaaaa tggaagggac atgtatgttg atcaggaact
      181 ggacataaac cgtttatctg attacgacgt cgatcacatt gtaccccaat cctttttgaa
      241 ggacgattca atcgacaata aagtgcttac acgctcggat aagaaccgag ggaaaagtga
      301 caatgttcca agcgaggaag tcgtaaagaa aatgaagaac tattggcggc agctcctaaa
      361 tgcgaaactg ataacgcaaa gaaagttcga taacttaact aaagctgaga ggggtggctt
      421 gtctgaactt gacaaggccg gatttattaa acgtcagctc gtggaaaccc gccaaatcac
      481 aaagcatgtt gcacagatac tagattcccg aatgaatacg aaatacgacg agaacgataa
      541 gctgattcgg gaagtcaaag taatcacttt aaagtcaaaa ttggtgtcgg acttcagaaa
      601 ggattttcaa ttctataaag ttagggagat aaataactac caccatgcgc acgacgctta
      661 tcttaatgcc gtcgtaggga ccgcactcat taagaaatac ccgaagctag aaagtga
//
