LOCUS       sequence-87775-e         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIbeta F 3-4
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(101..406)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(203..222)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 caatatatcc tgtcaaacac tgatagttta aaccagagaa gacaagacag acatgagacc
       61 gtcgacctgc agactggctg tgtataaggg agcctgacat ttatattccc cagaacatca
      121 ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga
      181 taacggaaac cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga
      241 tatgcaccac cgggtaaagt tcacggggga ctttatctga cagcagacgt gcactggcca
      301 gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca
      361 gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagcccctg
      421 ttctcgtcag caaaagagcc gttcatttca ataaaccggg cgacctcagc catcccttcc
      481 tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgtg
      541 cttaccagac cggagatatt gacatcatat atgccttgag caactgatag ctgtcgctgt
      601 caactgtcac tgtaatacgc tgcttcatag catacctctt tttgacatac ttcgggtata
      661 catatcagta tatattctta taccgcaaaa atcagcgcgc a
//
