LOCUS       sequence-87777-e         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIbeta R 4-5
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(109..414)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(211..230)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ttacccgcca atatatcctg tcaaacactg atagtttaaa ccagagaaga caactcagcg
       61 gtgagaccgt cgacctgcag actggctgtg tataagggag cctgacattt atattcccca
      121 gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc
      181 ttccccgata acggaaaccg gcacactggc catatcggtg gtcatcatgc gccagctttc
      241 atccccgata tgcaccaccg ggtaaagttc acgggggact ttatctgaca gcagacgtgc
      301 actggccagg gggatcacca tccgtcgccc gggcgtgtca ataatatcac tctgtacatc
      361 cacaaacaga cgataacggc tctctctttt ataggtgtaa accttaaact gcatttcacc
      421 agcccctgtt ctcgtcagca aaagagccgt tcatttcaat aaaccgggcg acctcagcca
      481 tcccttcctg attttccgct ttccagcgtt cggcacgcag acgacgggct tcattctgca
      541 tggttgtgct taccagaccg gagatattga catcatatat gccttgagca actgatagct
      601 gtcgctgtca actgtcactg taatacgctg cttcatagca tacctctttt tgacatactt
      661 cgggtataca tatcagtata tatt
//
