LOCUS       sequence-87779-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  wildtype Cas9 expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Tsai SQ, Wyvekens N, Khayter C, Foden JA, Thapar V, Reyon D, Goodwin
            MJ, Aryee MJ, Joung JK
  TITLE     Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome 
            editing.
  JOURNAL   Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908.
  PUBMED    24770325
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908."
COMMENT     Sequence Label: pSQT817
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(70..135)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(142..162)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cagggcattg gccacaccag ccaccacctt ctgataggca gcctgcacct gaggagtgcg
       61 gccgcttcac ttgtcatcgt catccttgta atcgatgtca tgatctttat aatcaccgtc
      121 atggtctttg tagtcgctcg agactttcct cttcttcttg ggggatccgt cacccccaag
      181 ctgtgacaaa tctatccgag tttcatataa tcccgtgatg gattggtgaa tcagtgtcgc
      241 gtctagcacc tccttggtag aagtgtatcg tttgcgatct atcgttgtgt caaaatactt
      301 gaatgcggct ggagcgccga ggttggtaag agtaaacaaa tggataatat tttccgcctg
      361 ctcacgtatg ggtttatccc tgtgcttgtt gtatgcgctt aatactttgt ccagattggc
      421 atcagctagg atgactctct tactgaattc cgaaatttgc tctatgattt cgtcgagata
      481 atgtttgtgc tgctcaacaa aaagttgctt ctgttcgtta tcttcaggtg aacctttcaa
      541 cttctcgtaa tgggacgcta aatacaggaa attcacgtat ttagacggta gtgcgagttc
      601 gttccccttt tgaagctctc cggcgctagc caacatccgt tttcggccat tttctaactc
      661 aaacagacta tactttggta gtttaattat gagatccttt tttacttcct tgtaaccttt
      721 cgcctcaagg aagtcgatgg ggttcttttc aaaaaa
//
