LOCUS       sequence-87780-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIbeta F 1-2
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    14..38
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(124..429)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(226..245)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaaccaga
       61 gaagacaagc ccgacatgag accgtcgacc tgcagactgg ctgtgtataa gggagcctga
      121 catttatatt ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct
      181 gagatcagcc acttcttccc cgataacgga aaccggcaca ctggccatat cggtggtcat
      241 catgcgccag ctttcatccc cgatatgcac caccgggtaa agttcacggg ggactttatc
      301 tgacagcaga cgtgcactgg ccagggggat caccatccgt cgcccgggcg tgtcaataat
      361 atcactctgt acatccacaa acagacgata acggctctct cttttatagg tgtaaacctt
      421 aaactgcatt tcaccagccc ctgttctcgt cagcaaaaga gccgttcatt tcaataaacc
      481 gggcgacctc agccatccct tcctgatttt ccgctttcca gcgttcggca cgcagacgac
      541 gggcttcatt ctgcatggtt gtgcttacca gaccggagat attgacatca tatatgcctt
      601 gagcaactga tagctgtcgc tgtcaactgt cactgtaata cgctgcttca tagcatacct
      661 ctttttgaca tacttcgggt atacatatca gtatatattc ttataccgca aaaatcagcg
      721 cgcaaatacg catactgtta tctggctttt agtaagccgg atcctctaga ttacgccccg
      781 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
      841 tcacaaacgg
//
