LOCUS       sequence-87781-e         443 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIbeta F 1-2
FEATURES             Location/Qualifiers
     source          1..443
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(134..139,140..157,158..164)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(284..303)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta
       61 cataatactg taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt
      121 aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta
      181 ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
      241 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
      301 tgctcaatgt acctataacc agaccgttca actggatatt acggcctttt taaagaccgt
      361 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
      421 tgctcatccg gaattccgta tgg
//
