LOCUS       sequence-87783-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIbeta F 2-3
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(88..393)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(190..209)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 caaacactga tagtttaaac cagagaagac aacaatgaca tgagaccgtc gacctgcaga
       61 ctggctgtgt ataagggagc ctgacattta tattccccag aacatcaggt taatggcgtt
      121 tttgatgtca ttttcgcggt ggctgagatc agccacttct tccccgataa cggaaaccgg
      181 cacactggcc atatcggtgg tcatcatgcg ccagctttca tccccgatat gcaccaccgg
      241 gtaaagttca cgggggactt tatctgacag cagacgtgca ctggccaggg ggatcaccat
      301 ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc acaaacagac gataacggct
      361 ctctctttta taggtgtaaa ccttaaactg catttcacca gcccctgttc tcgtcagcaa
      421 aagagccgtt catttcaata aaccgggcga cctcagccat cccttcctga ttttccgctt
      481 tccagcgttc ggcacgcaga cgacgggctt cattctgcat ggttgtgctt accagaccgg
      541 agatattgac atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt
      601 aatacgctgc ttcatagcat acctcttttt gacatacttc gggtatacat atcagtatat
      661 attcttatac cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc ttttagtaag
      721 ccggatcctc tagattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
      781 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
//
