LOCUS       sequence-87788-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI+Bpi
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    53..177
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(202..207,208..225,226..232)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(352..371)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gcatctagat atcggatccc gggcccgtcg actgcagagg tacgaagtct tcacaagttt
       61 gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat
      121 tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt cactatggcg
      181 gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt
      241 ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
      301 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
      361 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
      421 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
      481 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
      541 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
      601 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
      661 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
      721 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
      781 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
      841 ccgctggcga ttcaggttca tcatgccgtt
//
