LOCUS       sequence-87789-e         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI+Bpi
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..162
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(203..508)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(305..324)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ttacgccaag cttgcatgca ggcagaatgt cttcccaacc actttgtaca agaaagctga
       61 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      121 gactacataa tactgtaaaa cacaacatat ccagtcacta tggtcgacct gcagactggc
      181 tgtgtataag ggagcctgac atttatattc cccagaacat caggttaatg gcgtttttga
      241 tgtcattttc gcggtggctg agatcagcca cttcttcccc gataacggag accggcacac
      301 tggccatatc ggtggtcatc atgcgccagc tttcatcccc gatatgcacc accgggtaaa
      361 gttcacggga gactttatct gacagcagac gtgcactggc cagggggatc accatccgtc
      421 gcccgggcgt gtcaataata tcactctgta catccacaaa cagacgataa cggctctctc
      481 ttttataggt gtaaacctta aactgcattt caccagcccc tgttctcgtc agcaaaagag
      541 ccgttcattt caataaaccg ggcgacctca gccatccctt cctgattttc cgctttccag
      601 cgttcggcac gcagacgacg ggcttcattc tgcatggttg tgcttaccag accggagata
      661 ttgacatcat atatgccttg agcaactgat agctgtcgct gtcaactgtc actgtaatac
      721 gctgcttcat a
//
