LOCUS       sequence-8806-ex        3221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Pluta K, Luce MJ, Bao L, Agha-Mohammadi S, Reiser J
  TITLE     Tight control of transgene expression by lentivirus vectors 
            containing second-generation tetracycline-responsive promoters.
  JOURNAL   J Gene Med. 2005 Jun . 7(6):803-17.
  PUBMED    15655804
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gene Med.
            2005 Jun . 7(6):803-17."
COMMENT     Sequence Label: pLitTREPitt
FEATURES             Location/Qualifiers
     source          1..3221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        38..142
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(143..211,212..1003)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(361..380)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(1045..1554)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1240..1261)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1448..1467
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      1665..2253
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2154..2173
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    2314..2335
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2350..2355,2356..2373,2374..2380)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2388..2404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2393..2415
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2412..2428
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2412..2428
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2589..2613)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2635..2653
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2672..2690
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2709..2727
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2746..2764
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2783..2801
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2820..2838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2857..2875
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2894..2912
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(3022..3039)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3022..3038)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3031..3053)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gttaactacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
       61 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      121 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      181 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      241 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      301 gatccttgag agttttcgcc ccgaagaacg ttctccaatg atgagcactt ttaaagttct
      361 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
      421 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      481 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      541 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      601 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
      661 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
      721 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
      781 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
      841 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
      901 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
      961 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1021 ctcatatata ctttagattg atttaccccg gttgataatc agaaaagccc caaaaacagg
     1081 aagattgtat aagcaaatat ttaaattgta aacgttaata ttttgttaaa attcgcgtta
     1141 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     1201 aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     1261 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     1321 ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta
     1381 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcg aacgtggcga
     1441 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     1501 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtaaaagg
     1561 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     1621 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1681 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1741 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1801 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1861 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1921 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1981 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2041 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2101 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2161 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2221 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2281 ttcctggcct tttgctggcc ttttgctcac atgtaatgtg agttagctca ctcattaggc
     2341 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     2401 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctacgta atacgactca
     2461 ctagtgggta ccagagctcc ctaggttcta gaaccggtga cgtctcccat ggtgaagctt
     2521 ggatccaggc tggatcggtc ccggtgtctt ctatggaggt caaaacagcg tggatggcgt
     2581 ctccaggcga tctgacggtt cactaaacga gctctgctta tatagggggt accttctcta
     2641 tcactgatag ggagctcgag acgtgtcgac ttctctatca ctgataggga gacgttctag
     2701 acgtaccttc tctatcactg atagggagct cgagacgtgt cgacttctct atcactgata
     2761 gggagacgtt ctagacgtac cttctctatc actgataggg agctcgagac gtgtcgactt
     2821 ctctatcact gatagggaga cgttctagac gtaccttctc tatcactgat agggagctcg
     2881 agacgtgtcg acttctctat cactgatagg gagacgtcag gtggcacttg atatcctgca
     2941 ggaattcctc gagaccgtac gtgcgcgcga atgcatccag atcttccctc tagtcaaggc
     3001 cttaagtgag tcgtattacg gactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     3061 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     3121 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     3181 cttcgcttgg taataaagcc cgcttcggcg ggcttttttt t
//
