LOCUS       sequence-8811-ex        3495 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cloning vector containing attB flanked TXNL4B useful for 
            recombination based conversion of conventional vectors to Gateway 
            compatible destination vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Guo F, Chiang MY, Wang Y, Zhang YZ
  TITLE     An in vitro recombination method to convert restriction- and 
            ligation-independent expression vectors.
  JOURNAL   Biotechnol J. 2008 Mar . 3(3):370-7.
  PUBMED    18064608
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2008 Mar . 3(3):370-7."
COMMENT     Sequence Label: pBZ-attBtxnl4b
FEATURES             Location/Qualifiers
     source          1..3495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    17..38
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(53..58,59..76,77..83)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    91..107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     96..118
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     115..131
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     115..131
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     150..170
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        152..170
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    256..280
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(734..758)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(784..803)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(785..803)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(813..830)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(813..829)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(822..844)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      971..1426
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1058..1077)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1268..1289
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1500..1604
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1605..1673,1674..2465)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1823..1842)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2636..3224
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3125..3144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3378..3395
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
       61 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
      121 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
      181 tggagctcca ccgcggtggc ggccgctcta gagcatatga cgtcggcgcc atgggcggcc
      241 gctgaattca ttaatacaag tttgtacaaa aaagcaggct cgatgagctt cctcttgccc
      301 aaactgacca gcaagaaaga agtagaccag gcgatcaaaa gcactgcgga gaaggtgttg
      361 gttctcaggt ttgggaggga cgaggacccc gtctgtctgc agctagatga tattctttct
      421 aagacctctt ctgacttaag taaaatggct gctatatacc tggtagatgt ggaccaaact
      481 gcagtttata cacagtattt tgacatcagt tatattccat ctactgtctt tttcttcaat
      541 gggcagcaca tgaaagtgga ttatggatct ccagatcaca ctaagtttgt gggaagcttc
      601 aaaaccaaac aagacttcat agatttgatt gaagtaatct atcgaggagc aatgaggggg
      661 aagcttattg tccaaagtcc tattgatccc aagaatattc ccaaatatga ccttctctat
      721 caagacattt aggacccagc tttcttgtac aaagtggtcc tcgaggatcc ggtacccaat
      781 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
      841 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
      901 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
      961 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     1021 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     1081 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     1141 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     1201 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     1261 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     1321 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     1381 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttagtg atcgttggga
     1441 accggagctg aatgaagcca taccaaacga cgagcggtgg cacttttcgg ggaaatgtgc
     1501 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     1561 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     1621 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     1681 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     1741 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     1801 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     1861 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     1921 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     1981 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     2041 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     2101 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     2161 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     2221 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     2281 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     2341 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     2401 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     2461 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     2521 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     2581 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2641 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2701 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2761 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     2821 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2881 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2941 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3001 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3061 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3121 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3181 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3241 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3301 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3361 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     3421 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     3481 actggaaagc gggca
//
