LOCUS       sequence-8817-ex        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Jin J, Li X, Gygi SP, Harper JW
  TITLE     Dual E1 activation systems for ubiquitin differentially regulate E2 
            enzyme charging.
  JOURNAL   Nature. 2007 Jun 28. 447(7148):1135-8.
  PUBMED    17597759
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jun 28. 447(7148):1135-8."
COMMENT     Sequence Label: pDEST17-Cdc34a
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    797..808
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(853..877)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 anattttnnt taactttaag aaggagatat acatatgtcg tactaccatc accatcacca
       61 tcacctcgaa tcaacaagtt tgtacaaaaa agcaggctcc gcggccgccc ccttcaccat
      121 ggctcggccg ctagtgccca gctcgcagaa ggcgctgctg ctggagctca aggggctgca
      181 ggaagagccg gtcgagggat tccgcgtgac actggtggac gagggcgatc tatacaactg
      241 ggaggtggcc atcttcgggc cccccaacac ctactacgag ggcggctact tcaaggcgcg
      301 cctcaagttc cccatcgact acccatactc tccaccagcc tttcggttcc tgaccaagat
      361 gtggcaccct aacatctacg agacggggga cgtgtgtatc tccatcctcc acccgccggt
      421 ggacgacccc cagagcgggg agctgccctc agagaggtgg aaccccacgc agaacgtcag
      481 gaccattctc ctgagtgtga tctccctcct gaacgagccc aacaccttct cgcccgcaaa
      541 cgtggacgcc tccgtgatgt acaggaagtg gaaagagagc aaggggaagg atcgggagta
      601 cacagacatc atccggaagc aggtcctggg gaccaaggtg gacgcggagc gtgacggcgt
      661 gaaggtgccc accacgctgg ccgagtactg cgtgaagacc aaggcgccgg cgcccgacga
      721 gggctcagac ctcttctacg acgactacta cgaggacggc gaggtggagg angaggccga
      781 cagctgcttc ggggacgatg aggatgactc tggcacggag gantcctgac tcgaggaang
      841 gtgggcgcgc cgacccagct ttcttgtaca aagtggttga ttcnaggctg ctaacaaagc
      901 ccgaaaggaa gctganttgg ctgctgccnn cgctgancaa taactagcat nancccntgg
      961 ggcnnctaaa cgggtnntgn ggggtttttg ctgnangnnn nantnnntnc nnnatcnnnn
     1021 gganggnngn ngncnccntg atcnnnnann c
//
