LOCUS       sequence-88260-e         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Beta-Catenin-20
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      76..85
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             127..162
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgcggccac catggctact caagctgacc tgatggagtt ggacatggcc
      121 atggagccgg acagaaaagc tgctgtcagc cactggcagc agcagtctta cttggattct
      181 ggaatccatt ctggtgccac caccacagct ccttccctga gtggcaaggg caaccctgag
      241 gaagaagatg ttgacacctc ccaagtcctt tatgaatggg agcaaggctt ttcccagtcc
      301 ttcacgcaag agcaagtagc tgatattgac gggcagtatg caatgactag ggctcagagg
      361 gtccgagctg ccatgttccc tgagacgcta gatgagggca tgcagatccc atccacgcag
      421 tttgacgctg ctcatcccac taatgtccag cgcttggctg aaccatcaca gatgttgaaa
      481 catgcagttg tcaatttgat taactatcag gatgacgcgg aacttgccac acgtgcaatt
      541 cctgagctga caaaactgct aaacgatgag gaccaggtgg tagttaataa agctgctgtt
      601 atggtccatc agctttccaa aaaggaagct tccagacatg ccatcatgcg ctcccctcag
      661 atggtgtctg ccattgtacg caccatgcag aatacaaatg atgtagagac agctcgttgt
      721 actgctggga ctctgcacaa cctttctcac caccgcgagg gcttgctggc catctttaag
      781 tctggtggca tcccagcgct ggtgaaaatg cttgggtcac cagtggattc tgtactgttc
      841 tacgccatca cgacactgca taatctcctg ctccatcagg aaggagctaa aatggcagtg
      901 cgcctagctg gtggactgca gaaaatggtt gctttgctca acaaaacaaa cgtgaaattc
      961 ttggctatta caacagactg ccttcaga
//
