LOCUS       sequence-88268-e        1560 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tetraspanin/Membrane, Excitation: 400 / 504, Emission:
            515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1560)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPA-GFP-CD81-10
FEATURES             Location/Qualifiers
     source          1..1560
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(23..25,26..28,29..739)
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(68..89)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(329..348)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     676..697
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
       61 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
      121 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      181 gctgcccgtg ccctggccca ccctcgtgac caccttcagc tacggcgtgc agtgcttcag
      241 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      301 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
      361 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      421 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
      481 catggccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga
      541 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
      601 cgtgctgctg cccgacaacc actacctgag ccaccagtcc aagctgagca aagaccccaa
      661 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
      721 catggacgag ctgtacaagt ccggactcag atctcgagct caagcttccg gagtggaggg
      781 ctgcaccaag tgcatcaagt acctgctctt cgtcttcaat ttcgtcttct ggctggctgg
      841 aggcgtgatc ctgggtgtgg ccctgtggct ccgccatgac ccgcagacca ccaacctcct
      901 gtatctggag ctgggagaca agcccgcgcc caacaccttc tatgtaggca tctacatcct
      961 catcgctgtg ggcgctgtca tgatgttcgt tggcttcctg ggctgctacg gggccatcca
     1021 ggaatcccag tgcctgctgg ggacgttctt cacctgcctg gtcatcctgt ttgcctgtga
     1081 ggtggccgcc ggcatctggg gctttgtcaa caaggaccag atcgccaagg atgtgaagca
     1141 gttctatgac caggccctac agcaggccgt ggtggatgat gacgccaaca acgccaaggc
     1201 tgtggtgaag accttccacg agacgcttga ctgctgtggc tccagcacac tgactgcttt
     1261 gaccacctca gtgctcaaga acaatttgtg tccctcgggc agcaacatca tcagcaacct
     1321 cttcaaggag gactgccacc agaagatcga tgacctcttc tccgggaagc tgtacctcat
     1381 cggcattgct gccatcgtgg tcgctgtgat catgatcttc gagatgatcc tgagcatggt
     1441 gctgtgctgt ggcatccgga acagctccgt gtactgaggc ggatccaagg gcgaattctg
     1501 cagtcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc ataatcagcc
//
