LOCUS       sequence-88269-e        1603 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tetraspanin/Membrane, Excitation: 400 / 504, Emission:
            515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1603)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPA-GFP-CD151-7
FEATURES             Location/Qualifiers
     source          1..1603
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(29..31,32..34,35..745)
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(74..95)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(335..354)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     682..703
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
       61 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      121 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      181 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcagctacg gcgtgcagtg
      241 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      301 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      361 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      421 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
      481 ctatatcatg gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa
      541 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
      601 cggccccgtg ctgctgcccg acaaccacta cctgagccac cagtccaagc tgagcaaaga
      661 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
      721 tctcggcatg gacgagctgt acaagtccgg actcagatct ggcggtatgg gtgagttcaa
      781 cgagaagaag acaacatgtg gcaccgtttg cctcaagtac ctgctgttta cctacaattg
      841 ctgcttctgg ctggctggcc tggctgtcat ggcagtgggc atctggacgc tggccctcaa
      901 gagtgactac atcagcctgc tggcctcagg cacctacctg gccacagcct acatcctggt
      961 ggtggcgggc actgtcgtca tggtgactgg ggtcttgggc tgctgcgcca ccttcaagga
     1021 gcgtcggaac ctgctgcgcc tgtacttcat cctgctcctc atcatctttc tgctggagat
     1081 catcgctggt atcctcgcct acgcctacta ccagcagctg aacacggagc tcaaggagaa
     1141 cctgaaggac accatgacca agcgctacca ccagccgggc catgaggctg tgaccagcgc
     1201 tgtggaccag ctgcagcagg agttccactg ctgtggcagc aacaactcac aggactggcg
     1261 agacagtgag tggatccgct cacaggaggc cggtggccgt gtggtcccag acagctgctg
     1321 caagacggtg gtggctcttt gtggacagcg agaccatgcc tccaacatct acaaggtgga
     1381 gggcggctgc atcaccaagt tggagacctt catccaggag cacctgaggg tcattggggc
     1441 tgtggggatc ggcattgcct gtgtgcaggt ctttggcatg atcttcacgt gctgcctgta
     1501 caggagtctc aagctggagc actactgaga attctgcagt cgacggtacc gcgggcccgg
     1561 gatccaccgg atctagataa ctgatcataa tcagccatac cac
//
