LOCUS       sequence-88273-e        1687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 400 / 504, 
            Emission: 515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1687)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPA-GFP-EB3-7
FEATURES             Location/Qualifiers
     source          1..1687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      61..70
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      925..934
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(931..933,934..936,937..1647)
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(976..997)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1237..1256)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1584..1605
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattct gcagtcgact
       61 gccaccatgg ccgtcaatgt gtactccaca tctgtgacca gtgaaaatct gagtcgccat
      121 gatatgcttg catgggtcaa cgactccctg cacctcaact ataccaagat agaacagctt
      181 tgttcagggg cagcctactg ccagttcatg gacatgctct tccccggctg tgtgcacttg
      241 aggaaagtga agttccaggc caaactagag catgaataca tccacaactt caaggtgctg
      301 caagcagctt tcaagaagat gggtgttgac aaaatcattc ctgtagagaa attagtgaaa
      361 ggaaaattcc aagataattt tgagtttatt cagtggttta agaaattctt tgacgcaaac
      421 tatgatggaa aggattacaa ccctctgctg gcgcggcagg gccaggacgt agcgccacct
      481 cctaacccag gtgatcagat cttcaacaaa tccaagaaac tcattggcac agcagttcca
      541 cagaggacgt cccccacagg cccaaaaaac atgcagacct ctggccggct gagcaatgtg
      601 gcccccccct gcattctccg gaagaatcct ccatcagccc gaaatggcgg ccatgagact
      661 gatgcccaaa ttcttgaact caaccaacag ctggtggact tgaagctgac agtggatggg
      721 ctggagaagg aacgtgactt ctacttcagc aaacttcgtg acatcgagct catctgccag
      781 gagcatgaaa gtgaaaacag ccctgttatc tcaggcatca ttggcatcct ctatgccaca
      841 gaggaaggat tcgcaccccc tgaggacgat gagattgaag agcatcaaca agaagaccag
      901 gacgagtacc tggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
      961 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     1021 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     1081 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcagcta cggcgtgcag
     1141 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1201 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1261 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1321 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     1381 gtctatatca tggccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac
     1441 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     1501 gacggccccg tgctgctgcc cgacaaccac tacctgagcc accagtccaa gctgagcaaa
     1561 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1621 actctcggca tggacgagct gtacaagtaa agcggccgcg actctagatc ataatcagcc
     1681 ataccac
//
