LOCUS       sequence-88289-e        2042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 400 / 504, Emission: 515 / 
            517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2042)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPA-GFP-MAP4-C-10
FEATURES             Location/Qualifiers
     source          1..2042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(28..30,31..33,34..744)
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(73..94)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(334..353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     681..702
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcagctacgg cgtgcagtgc
      241 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
      481 tatatcatgg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
      541 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagccacc agtccaagct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      721 ctcggcatgg acgagctgta caagtccgga ctcagatctg gtggctccgc aggatcccgg
      781 caagaagaag caaaggctgc tgtaggtgtg actggaaatg acatcactac cccgccaaac
      841 aaggagccac caccaagccc agaaaagaaa gcaaagcctt tggccaccac tcaacctgca
      901 aagacttcaa catcgaaagc caaaacacag cccacttctc tccctaagca accagctccc
      961 accacctctg gtgggttgaa taaaaaaccc atgagcctcg cctcaggctc agtgccagct
     1021 gccccacaca aacgccctgc tgctgccact gctactgcca ggccttccac cctacctgcc
     1081 agagacgtga agccaaagcc aattacagaa gctaaggttg ccgaaaagcg gacctctcca
     1141 tccaagcctt catctgcccc agccctcaaa cctggaccta aaaccacccc aaccgtttca
     1201 aaagccacat ctccctcaac tcttgtttcc actggaccaa gtagtagaag tccagctaca
     1261 actctgccta agaggccaac cagcatcaag actgagggga aacctgctga tgtcaaaagg
     1321 atgactgcta agtctgcctc agctgacttg agtcgctcaa agaccacctc tgccagttct
     1381 gtgaagagaa acaccactcc cactggggca gcacccccag cagggatgac ttccactcga
     1441 gtcaagccca tgtctgcacc tagccgctct tctggggctc tttctgtgga caagaagccc
     1501 acttccacta agcctagctc ctctgctccc agggtgagcc gcctggccac aactgtttct
     1561 gcccctgacc tgaagagtgt tcgctccaag gtcggctcta cagaaaacat caaacaccag
     1621 cctggaggag gccgggccaa ggtagagaaa aaaacagagg cagctaccac agctgggaag
     1681 cctgaaccta atgcagtcac taaagcagcc ggctccattg cgagtgcaca gaaaccgcct
     1741 gctgggaaag tccagatagt atccaaaaaa gtgagctaca gtcatattca atccaagtgt
     1801 gtttccaagg acaatattaa gcatgtccct ggatgtggca atgttcagat tcagaacaag
     1861 aaagtggaca tatccaaggt ctcctccaag tgtgggtcca aagctaatat caagcacaag
     1921 cctggtggag gagatgtcaa gattgaaagt cagaagttga acttcaagga gaaggcccaa
     1981 gccaaagtgg gatagggatc caccggatct agataactga tcataatcag ccataccaca
     2041 tt
//
