LOCUS       sequence-88301-e        1404 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Talin Actin Binding Module, Excitation: 400 / 504, 
            Emission: 515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1404)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPA-GFP-Talin-ABM-10
FEATURES             Location/Qualifiers
     source          1..1404
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(27..29,30..32,33..743)
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(72..93)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(333..352)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     680..701
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
       61 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      121 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      181 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cagctacggc gtgcagtgct
      241 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      301 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      361 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      421 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
      481 atatcatggc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca
      541 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      601 gccccgtgct gctgcccgac aaccactacc tgagccacca gtccaagctg agcaaagacc
      661 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
      721 tcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcgatcctag
      781 aagctgccaa gtccatcgct gcagccacca gtgcactggt aaaggctgcg tcagcagccc
      841 agagggagct ggtggctcaa ggaaaggtgg gcgccattcc agccaatgca ctggatgatg
      901 ggcagtggtc gcagggcctc atttccgctg cccgtatggt ggctgcggcc accaacaatc
      961 tgtgtgaagc agctaacgca gctgtccagg gccatgctag ccaggagaaa ctcatttcct
     1021 cagccaagca agtagctgcc tccacagctc agctcctggt agcttgcaag gtcaaagcag
     1081 atcaggactc tgaggcaatg aaacggctcc aggctgctgg caatgcagtg aagagggcct
     1141 cagataacct ggtaaaggcg gcccagaaag ctgcagcctt cgaagaccag gagaatgaga
     1201 cggtggtggt gaaggagaaa atggttgggg gcattgccca gattatcgca gcacaggaag
     1261 agatgcttcg gaaggaacga gagctggaag aggctcggaa aaagctcgcc cagatccggc
     1321 agcagcagta caagttcttg ccttcagagc ttcgagacga gcactaagga tccaccggat
     1381 ctagataact gatcataatc agcc
//
