LOCUS       sequence-88302-e        1234 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Outer Mitochondrial Membrane, Excitation: 400 / 504, 
            Emission: 515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1234)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPA-GFP-TOMM20-N-10
FEATURES             Location/Qualifiers
     source          1..1234
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      3..12
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      468..477
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(474..476,477..479,480..1190)
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(519..540)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(780..799)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1127..1148
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcgccaccat ggtgggtcgg aacagcgcca tcgccgccgg tgtatgcggg gcccttttca
       61 ttgggtactg catctacttc gaccgcaaaa gacgaagtga ccccaacttc aagaacaggc
      121 ttcgagaacg aagaaagaaa cagaagcttg ccaaggagag agctgggctt tccaagttac
      181 ctgaccttaa agatgctgaa gctgttcaga agttcttcct tgaagaaata cagcttggtg
      241 aagagttact agctcaaggt gaatatgaga agggcgtaga ccatctgaca aatgcaattg
      301 ctgtgtgtgg acagccacag cagttactgc aggtcttaca gcaaactctt ccaccaccag
      361 tgttccagat gcttctgact aagctcccaa caattagtca gagaattgta agtgctcaga
      421 gcttggctga agatgatgtg gaaggcggta gcggggatcc accggtcgcc accatggtga
      481 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      541 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      601 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      661 ccaccttcag ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
      721 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
      781 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
      841 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
      901 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
      961 aggccaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     1021 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1081 gccaccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1141 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc
     1201 gcgactctag atcataatca gccataccac attt
//
