LOCUS       sequence-88305-e        2210 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 400 / 504, Emission: 515 / 
            517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Day RN, Davidson MW
  TITLE     The fluorescent protein palette: tools for cellular imaging.
  JOURNAL   Chem Soc Rev. 2009 Oct;38(10):2887-921. doi: 10.1039/b901966a. Epub 
            2009 Aug 4.
  PUBMED    19771335
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1039/b901966a"; 
            journalName: "Chem Soc Rev"; date: "2009-10"; volume: "38"; issue: 
            "10"; pages: "2887-921"
COMMENT     Sequence Label: mPA-GFP-Tubulin-C-18
FEATURES             Location/Qualifiers
     source          1..2210
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(37..39,40..42,43..753)
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(82..103)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(343..362)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     690..711
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtgacgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg
       61 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      121 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      181 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cagctacggc
      241 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      301 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      361 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
      421 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
      481 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggccaa cttcaagatc
      541 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
      601 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagccacca gtccaagctg
      661 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
      721 gggatcactc tcggcatgga cgagctgtac aagtccggac tcagatctgg cagcggtgga
      781 ggcagcgcat ccggcggaag cggaagcgtg cgtgagtgca tctccatcca cgttggccag
      841 gctggtgtcc agattggcaa tgcctgctgg gagctctact gcctggaaca cggcatccag
      901 cccgatggcc agatgccaag tgacaagacc attgggggag gagatgactc cttcaacacc
      961 ttcttcagtg agacgggcgc tggcaagcac gtgccccggg ctgtgtttgt agacttggaa
     1021 cccacagtca ttgatgaagt tcgcactggc acctaccgcc agctcttcca ccctgagcag
     1081 ctcatcacag gcaaggaaga tgctgccaat aactatgccc gagggcacta caccattggc
     1141 aaggagatca ttgaccttgt gttggaccga attcgcaagc tggctgacca gtgcaccggt
     1201 cttcagggct tcttggtttt ccacagcttt ggtgggggaa ctggttctgg gttcacctcc
     1261 ctgctcatgg aacgtctctc agttgattat ggcaagaagt ccaagctgga gttctccatt
     1321 tacccagcac cccaggtttc cacagctgta gttgagccct acaactccat cctcaccacc
     1381 cacaccaccc tggagcactc tgattgtgcc ttcatggtag acaatgaggc catctatgac
     1441 atctgtcgta gaaacctcga tatcgagcgc ccaacctaca ctaaccttaa ccgccttatt
     1501 agccagattg tgtcctccat cactgcttcc ctgagatttg atggagccct gaatgttgac
     1561 ctgacagaat tccagaccaa cctggtgccc tacccccgca tccacttccc tctggccaca
     1621 tatgcccctg tcatctctgc tgagaaagcc taccatgaac agctttctgt agcagagatc
     1681 accaatgctt gctttgagcc agccaaccag atggtgaaat gtgaccctcg ccatggtaaa
     1741 tacatggctt gctgcctgtt gtaccgtggt gacgtggttc ccaaagatgt caatgctgcc
     1801 attgccacca tcaaaaccaa gcgcagcatc cagtttgtgg attggtgccc cactggcttc
     1861 aaggttggca tcaactacca gcctcccact gtggtgcctg gtggagacct ggccaaggta
     1921 cagagagctg tgtgcatgct gagcaacacc acagccattg ctgaggcctg ggctcgcctg
     1981 gaccacaagt ttgacctgat gtatgccaag cgtgcctttg ttcactggta cgtgggtgag
     2041 gggatggagg aaggcgagtt ttcagaggcc cgtgaagata tggctgccct tgagaaggat
     2101 tatgaggagg ttggtgtgga ttctgttgaa ggagagggtg aggaagaagg agaggaatac
     2161 taaggatcca ccggatctag ataactgatc ataatcagcc ataccacatt
//
