LOCUS       sequence-88306-e        1917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 400 / 504, 
            Emission: 515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1917)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPA-GFP-VASP-5
FEATURES             Location/Qualifiers
     source          1..1917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(36..38,39..41,42..752)
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(81..102)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(342..361)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     689..710
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gaccgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt
       61 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      121 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      181 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc agctacggcg
      241 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      301 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      361 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      421 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
      481 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
      541 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
      601 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagccaccag tccaagctga
      661 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
      721 ggatcactct cggcatggac gagctgtaca agtccggact cagatctatg agcgagacgg
      781 tcatctgttc cagccgggct actgtgatgc tttatgatga cagcaacaag cgatggctcc
      841 ctgctggcac tggtccccag gccttcagcc gcgtccagat ataccacaac cccactgcta
      901 actccttccg agttgttggc cgcaagatgc agccggatca gcaggtggtt atcaactgtg
      961 ccatcattcg gggtgtcaag tacaatcagg ccactcccat cttccatcag tggcgagatg
     1021 cccgccaggt ctggggcctc aacttcggca gcaaggagga cgccatacag tttgcaacag
     1081 gcatggccaa cgccctagag gccttggaag gaggtgggcc tcccccagcc ccagcacccc
     1141 ctgcctggtc tgcccagaat ggtccctccc cagaggagct ggaacaacag aaaaggcagc
     1201 cggagcatat ggagcgccgg gtctccaatg caggaggccc acctgctccc ccagctgggg
     1261 gccctcctcc acctccagga cctccccctc ctccaggtcc ccccccaccc ccaggtctgc
     1321 cctcctcagg ggtatctggg gcaggtcatg gagcaggggc agccccaccc cctgcacccc
     1381 cactccctac aacacagggc cccaatagtg ggggttccgg ggccccaggc ctggctgctg
     1441 ccattgctgg agccaaactc aggaaagtga gcaagcagga ggaggcctct ggggggcccc
     1501 tggcccccaa agctgagaac agtcgaagca ctggtggggg gcttatggaa gagatgaacg
     1561 ccatgctggc ccggagaaga aaagccacac aggttgggga gaagcccccc aaagacgagt
     1621 cagccagtga ggagtcagag gcccgactcc ctgcccagag tgaacctgtg agaagaccct
     1681 gggagaagaa cagcacaacc ttgccaagga tgaagtcgtc ctcttctgtg actacctccg
     1741 aggcccaccc ctcgacgccc tgctccagtg atgactccga cttggagagg gtgaagcagg
     1801 agcttctgga agaggtgcgg aaggagctac agaaaatgaa agaggaaatc atcgaagtct
     1861 ttgtccagga gctgaggaag cggggttctc cttgagcggc cgcgactcta gatcata
//
