LOCUS       sequence-88308-e        2380 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi/ER Transport, Excitation: 400 / 504, Emission: 
            515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2380)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPA-GFP-VSVG-12
FEATURES             Location/Qualifiers
     source          1..2380
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(47..94,95..1579)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein, 
                     thermosensitive tsO45 mutant"
                     /label=VSV-G tsO45
                     /note="thermosensitive variant of VSV-G Orsay"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHKDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAAIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNDICPTVHNSTTWHSDYKVKGLCDSNLISMDITSFSEDGELSSLGKEGTGFR
                     SNYFAYETGDKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPVFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDETLFFFGDTGLSKNPIEFVEGW
                     FSSWKSSIASFFFIIGLIIGLFLVLRVGIYLCIKLKHTKKRQIYTDIEMNRLG"
     CDS             join(1622..1624,1625..1627,1628..2338)
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1667..1688)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1928..1947)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2275..2296
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgcc gccaccatga agtgcctttt
       61 gtacttagct tttttattca tcggggtgaa ttgcaagttc accatagttt ttccacacaa
      121 ccaaaaagga aactggaaaa atgttccttc caattaccat tattgcccgt caagctcaga
      181 tttaaattgg cataaggact taataggcac agccttacaa gtcaaaatgc ccaagagtca
      241 caaggctatt caagcagacg gttggatgtg tcatgcttcc aaatgggtca ctacttgtga
      301 tttccgctgg tacggaccga agtatataac acattccatc cgatccttca ctccatctgt
      361 agaacaatgc aaggaaagca ttgaacaaac gaaacaagga acttggctga atccaggctt
      421 ccctcctcaa agttgtggat atgcaactgt gacggatgct gaagcagcga ttgtccaggt
      481 gactcctcac catgtgcttg ttgatgaata cacaggagaa tgggttgatt cacagttcat
      541 caacggaaaa tgcagcaatg acatatgccc cactgtccat aactccacaa cctggcattc
      601 cgactataag gtcaaagggc tatgtgattc taacctcatt tccatggaca tcacctcctt
      661 ctcagaggac ggagagctat catccctagg aaaggagggc acagggttca gaagtaacta
      721 ctttgcttat gaaaccggag acaaggcctg caaaatgcag tactgcaagc attggggagt
      781 cagactccca tcaggtgtct ggttcgagat ggctgataag gatctctttg ctgcagccag
      841 attccctgaa tgcccagaag ggtcaagtat ctctgctcca tctcagacct cagtggatgt
      901 aagtctcatt caggacgttg agaggatctt ggattattcc ctctgccaag aaacctggag
      961 caaaatcaga gcgggtcttc ccatctctcc agtggatctc agctatcttg ctcctaaaaa
     1021 cccaggaacc ggtcctgtct ttaccataat caatggtacc ctaaaatact ttgagaccag
     1081 atacatcaga gtcgatattg ctgctccaat cctctcaaga atggtcggaa tgatcagtgg
     1141 aactaccaca gaaagggaac tgtgggatga ctgggctcca tatgaagacg tggaaattgg
     1201 acccaatgga gttctgagga ccagttcagg atataagttt cctttatata tgattggaca
     1261 tggtatgttg gactccgatc ttcatcttag ctcaaaggct caggtgtttg aacatcctca
     1321 cattcaagac gctgcttcgc agcttcctga tgatgagact ttattttttt ttggtgatac
     1381 tgggctatcc aaaaatccaa tcgagtttgt agaaggttgg ttcagtagtt ggaagagctc
     1441 tattgcctct tttttcttta tcatagggtt aatcattgga ctattcttgg ttctccgagt
     1501 tggtatttat ctttgcatta aattaaagca caccaagaaa agacagattt atacagacat
     1561 agagatgaac cgacttggaa acagaattct gcagtcgacg gtaccgcggg cccgggatcc
     1621 aatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     1681 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     1741 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     1801 cctcgtgacc accttcagct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     1861 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     1921 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     1981 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     2041 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     2101 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     2161 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     2221 ctacctgagc caccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     2281 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     2341 aagcggccgc gactctagat cataatcagc cataccacat
//
