LOCUS       sequence-8831-ex        9772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pShuttle-CMV converted Gateway Destination vector for AdEasy 
            adenoviral vector system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9772)
  AUTHORS   Guo F, Chiang MY, Wang Y, Zhang YZ
  TITLE     An in vitro recombination method to convert restriction- and 
            ligation-independent expression vectors.
  JOURNAL   Biotechnol J. 2008 Mar . 3(3):370-7.
  PUBMED    18064608
REFERENCE   2  (bases 1 to 9772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2008 Mar . 3(3):370-7."
COMMENT     Sequence Label: pEZYshuttle
FEATURES             Location/Qualifiers
     source          1..9772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..103
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     enhancer        398..701
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        702..905
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     856..876
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1008..1132
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1561..1866)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(1663..1682)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(2211..2870)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2785..2804
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2871..2973)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(3151..3275)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    3435..3556
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3472..3491)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3526..3545
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6732..6834
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(6923..6940)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7094..7682)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7174..7193)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8514..9308
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8514..9308
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8568..8587)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9178..9197
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(9356..9735)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9418..9439)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9630..9649
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa nnnntaatag taatcaatta
      361 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      421 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      481 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      541 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      601 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      661 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      721 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      781 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      841 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      901 gagctggttt agtgaaccgt cagatccgct agagatctgg taccgtcgac gcggccgctc
      961 tagagcatat gacgtcggcg ccatgggcgg ccgctgaatt cattaataca agtttgtaca
     1021 aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc
     1081 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tgaatcaact
     1141 acttagatgg tattagtgac ctgtagtcga ccgacagcct tccaaatgtt cttcgggtga
     1201 tgctgccaac ttagtcgacc gacagccttc caaatgttct tctcaaacgg aatcgtcgta
     1261 tccagcctac tcgctattgt cctcaatgcc gtattaaatc ataaaaagaa ataagaaaaa
     1321 gaggtgcgag cctctttttt gtgtgacaaa ataaaaacat ctacctattc atatacgcta
     1381 gtgtcatagt cctgaaaatc atctgcatca agaacaattt cacaactctt atacttttct
     1441 cttacaagtc gttcggcttc atctggattt tcagcctcta tacttactaa acgtgataaa
     1501 gtttctgtaa tttctactgt atcgacctgc agactggctg tgtataaggg agcctgacat
     1561 ttatattccc cagaacatca ggttaatggc gtttttgatg tcattttcgc ggtggctgag
     1621 atcagccact tcttccccga taacggagac cggcacactg gccatatcgg tggtcatcat
     1681 gcgccagctt tcatccccga tatgcaccac cgggtaaagt tcacgggaga ctttatctga
     1741 cagcagacgt gcactggcca gggggatcac catccgtcgc ccgggcgtgt caataatatc
     1801 actctgtaca tccacaaaca gacgataacg gctctctctt ttataggtgt aaaccttaaa
     1861 ctgcatttca ccagcccctg ttctcgtcag caaaagagcc gttcatttca ataaaccggg
     1921 cgacctcagc catcccttcc tgattttccg ctttccagcg ttcggcacgc agacgacggg
     1981 cttcattctg catggttgtg cttaccagac cggagatatt gacatcatat atgccttgag
     2041 caactgatag ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag catacctctt
     2101 tttgacatac ttcgggtata catatcagta tatattctta taccgcaaaa atcagcgcgc
     2161 aaatacgcat actgttatct ggcttttagt aagccggatc cacgcggcgt ttacgccccg
     2221 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
     2281 tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     2341 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     2401 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac
     2461 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     2521 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     2581 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     2641 attgccatac ggaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     2701 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     2761 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     2821 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     2881 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
     2941 tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc
     3001 cagggcttcc cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc
     3061 gtcacaggta tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt agtcgactac
     3121 aggtcactaa taccatctaa gtagttgatt catagtgact ggatatgttg tgttttacag
     3181 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
     3241 tacgtttctc gttcagcttt cttgtacaaa gtggtcctcg agcctaagct tctagataag
     3301 atatccgatc caccggatct agataactga tcataatcag ccataccaca tttgtagagg
     3361 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     3421 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     3481 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     3541 tcatcaatgt atcttaacgc nnnntaaggg tgggaaagaa tatataaggt gggggtctta
     3601 tgtagttttg tatctgtttt gcagcagccg ccgccgccat gagcaccaac tcgtttgatg
     3661 gaagcattgt gagctcatat ttgacaacgc gcatgccccc atgggccggg gtgcgtcaga
     3721 atgtgatggg ctccagcatt gatggtcgcc ccgtcctgcc cgcaaactct actaccttga
     3781 cctacgagac cgtgtctgga acgccgttgg agactgcagc ctccgccgcc gcttcagccg
     3841 ctgcagccac cgcccgcggg attgtgactg actttgcttt cctgagcccg cttgcaagca
     3901 gtgcagcttc ccgttcatcc gcccgcgatg acaagttgac ggctcttttg gcacaattgg
     3961 attctttgac ccgggaactt aatgtcgttt ctcagcagct gttggatctg cgccagcagg
     4021 tttctgccct gaaggcttcc tcccctccca atgcggttta aaacataaat aaaaaaccag
     4081 actctgtttg gatttggatc aagcaagtgt cttgctgtct ttatttaggg gttttgcgcg
     4141 cgcggtaggc ccgggaccag cggtctcggt cgttgagggt cctgtgtatt ttttccagga
     4201 cgtggtaaag gtgactctgg atgttcagat acatgggcat aagcccgtct ctggggtgga
     4261 ggtagcacca ctgcagagct tcatgctgcg gggtggtgtt gtagatgatc cagtcgtagc
     4321 aggagcgctg ggcgtggtgc ctaaaaatgt ctttcagtag caagctgatt gccaggggca
     4381 ggcccttggt gtaagtgttt acaaagcggt taagctggga tgggtgcata cgtggggata
     4441 tgagatgcat cttggactgt atttttaggt tggctatgtt cccagccata tccctccggg
     4501 gattcatgtt gtgcagaacc accagcacag tgtatccggt gcacttggga aatttgtcat
     4561 gtagcttaga aggaaatgcg tggaagaact tggagacgcc cttgtgacct ccaagatttt
     4621 ccatgcattc gtccataatg atggcaatgg gcccacgggc ggcggcctgg gcgaagatat
     4681 ttctgggatc actaacgtca tagttgtgtt ccaggatgag atcgtcatag gccattttta
     4741 caaagcgcgg gcggagggtg ccagactgcg gtataatggt tccatccggc ccaggggcgt
     4801 agttaccctc acagatttgc atttcccacg ctttgagttc agatgggggg atcatgtcta
     4861 cctgcggggc gatgaagaaa acggtttccg gggtagggga gatcagctgg gaagaaagca
     4921 ggttcctgag cagctgcgac ttaccgcagc cggtgggccc gtaaatcaca cctattaccg
     4981 ggtgcaactg gtagttaaga gagctgcagc tgccgtcatc cctgagcagg ggggccactt
     5041 cgttaagcat gtccctgact cgcatgtttt ccctgaccaa atccgccaga aggcgctcgc
     5101 cgcccagcga tagcagttct tgcaaggaag caaagttttt caacggtttg agaccgtccg
     5161 ccgtaggcat gcttttgagc gtttgaccaa gcagttccag gcggtcccac agctcggtca
     5221 cctgctctac ggcatctcga tccagcatat ctcctcgttt cgcgggttgg ggcggctttc
     5281 gctgtacggc agtagtcggt gctcgtccag acgggccagg gtcatgtctt tccacgggcg
     5341 cagggtcctc gtcagcgtag tctgggtcac ggtgaagggg tgcgctccgg gctgcgcgct
     5401 ggccagggtg cgcttgaggc tggtcctgct ggtgctgaag cgctgccggt cttcgccctg
     5461 cgcgtcggcc aggtagcatt tgaccatggt gtcatagtcc agcccctccg cggcgtggcc
     5521 cttggcgcgc agcttgccct tggaggaggc gccgcacgag gggcagtgca gacttttgag
     5581 ggcgtagagc ttgggcgcga gaaataccga ttccggggag taggcatccg cgccgcaggc
     5641 cccgcagacg gtctcgcatt ccacgagcca ggtgagctct ggccgttcgg ggtcaaaaac
     5701 caggtttccc ccatgctttt tgatgcgttt cttacctctg gtttccatga gccggtgtcc
     5761 acgctcggtg acgaaaaggc tgtccgtgtc cccgtataca gacttgagag ggagtttaaa
     5821 cgaattcaat agcttgttgc atgggcggcg atataaaatg caaggtgctg ctcaaaaaat
     5881 caggcaaagc ctcgcgcaaa aaagaaagca catcgtagtc atgctcatgc agataaaggc
     5941 aggtaagctc cggaaccacc acagaaaaag acaccatttt tctctcaaac atgtctgcgg
     6001 gtttctgcat aaacacaaaa taaaataaca aaaaaacatt taaacattag aagcctgtct
     6061 tacaacagga aaaacaaccc ttataagcat aagacggact acggccatgc cggcgtgacc
     6121 gtaaaaaaac tggtcaccgt gattaaaaag caccaccgac agctcctcgg tcatgtccgg
     6181 agtcataatg taagactcgg taaacacatc aggttgattc atcggtcagt gctaaaaagc
     6241 gaccgaaata gcccggggga atacataccc gcaggcgtag agacaacatt acagccccca
     6301 taggaggtat aacaaaatta ataggagaga aaaacacata aacacctgaa aaaccctcct
     6361 gcctaggcaa aatagcaccc tcccgctcca gaacaacata cagcgcttca cagcggcagc
     6421 ctaacagtca gccttaccag taaaaaagaa aacctattaa aaaaacacca ctcgacacgg
     6481 caccagctca atcagtcaca gtgtaaaaaa gggccaagtg cagagcgagt atatatagga
     6541 ctaaaaaatg acgtaacggt taaagtccac aaaaaacacc cagaaaaccg cacgcgaacc
     6601 tacgcccaga aacgaaagcc aaaaaaccca caacttcctc aaatcgtcac ttccgttttc
     6661 ccacgttacg taacttccca ttttaagaaa actacaattc ccaacacata caagttactc
     6721 cgccctaaaa cctacgtcac ccgccccgtt cccacgcccc gcgccacgtc acaaactcca
     6781 ccccctcatt atcatattgg cttcaatcca aaataaggta tattattgat gatgttaatt
     6841 aacatgcatg gatccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     6901 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6961 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7021 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7081 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7141 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7201 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7261 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7321 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7381 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7441 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7501 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     7561 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7621 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7681 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7741 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7801 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7861 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7921 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7981 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8041 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8101 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8161 ccattgctgc agccatgaga ttatcaaaaa ggatcttcac ctagatcctt ttcacgtaga
     8221 aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga
     8281 caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat
     8341 agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct
     8401 ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg ccaaggatct
     8461 gatggcgcag gggatcaagc tctgatcaag agacaggatg aggatcgttt cgcatgattg
     8521 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     8581 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     8641 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
     8701 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     8761 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     8821 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     8881 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     8941 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     9001 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
     9061 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     9121 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     9181 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     9241 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     9301 tcttctgaat tttgttaaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     9361 atcggcaaca tcccttataa atcaaaagaa tagaccgcga tagggttgag tgttgttcca
     9421 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     9481 gtctatcagg gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttgcggtcg
     9541 aggtgccgta aagctctaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     9601 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     9661 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgcgcg cttaatgcgc
     9721 cgctacaggg cgcgtccatt cgccattcag gatcgaatta attcttaatt aa
//
