LOCUS       sequence-88312-e        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Alpha Actinin, Excitation: 400 / 504, Emission: 515 / 
            517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-Alpha-Actinin-19
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      295..304
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             301..1011
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(446..464)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ttcattgact tcatgtcccg cgagacagcc gacacagata cagcagacca agtcatggct
       61 tccttcaaga tcctggctgg ggacaagaac tacattacca tggacgagct gcgccgcgag
      121 ctgccacccg accaggctga gtactgcatc gcgcggatgg ccccctacac cggccccgac
      181 tccgtgccag gtgctctgga ctacatgtcc ttctccacgg cgctgtacgg cgagagtgac
      241 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcgccacc
      301 atggtgagca agggcgagga ggataacatg gccatcatta aggagttcat gcgcttcaag
      361 gtgcacatgg aggggtccgt gaacggccac gtgttcgaga tcgagggcga gggcgagggc
      421 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
      481 ttcacctggg acatcctgag ccctcagttc atgtacggct ccaatgccta cgtgaagcac
      541 cccgccgaca tccccgacta ctttaagctg tccttccccg agggcttcaa gtgggagcgc
      601 gtgatgaaat tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      661 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgtg
      721 atgcagaaga agaccatggg ctgggaggcc ctctccgagc ggatgtaccc cgaggacggc
      781 gccctgaagg gcgaggtcaa gcccagagtg aagctgaagg acggcggcca ctacgacgct
      841 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
      901 aaccgcaagc tggacatcac cagccacaac gaggactaca ccatcgtgga gcagtacgag
      961 agagccgagg gccgccactc caccggcggc atggacgagc tgtacaagta agcggccgcg
     1021 actctagatc ataatcagcc ataccacatt
//
