LOCUS       sequence-88315-e        2572 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 400 / 504, 
            Emission: 515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2572)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-Calnexin-N-14
FEATURES             Location/Qualifiers
     source          1..2572
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1843..1852
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1849..2559
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1994..2012)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gcgctaccgg actcagatct cgagaccgcc atggaaggga agtggttgct gtgtatgtta
       61 ctggtgcttg gaactgctat tgttgaggct catgatggac atgatgatga tgtgattgat
      121 attgaggatg accttgacga tgtcattgaa gaggtagaag actcaaaacc agataccact
      181 gctcctcctt catctcccaa ggttacttac aaagctccag ttccaacagg ggaagtatat
      241 tttgctgatt cttttgacag aggaactctg tcagggtgga ttttatccaa agccaagaaa
      301 gacgataccg atgatgaaat tgccaaatat gatggaaagt gggaggtaga ggaaatgaag
      361 gagtcaaagc ttccaggtga taaaggactt gtgttgatgt ctcgggccaa gcatcatgcc
      421 atctctgcta aactgaacaa gcccttcctg tttgacacca agcctctcat tgttcagtat
      481 gaggttaatt tccaaaatgg aatagaatgt ggtggtgcct atgtgaaact gctttctaaa
      541 acaccagaac tcaacctgga tcagttccat gacaagaccc cttatacgat tatgtttggt
      601 ccagataaat gtggagagga ctataaactg cacttcatct tccgacacaa aaaccccaaa
      661 acgggtatct atgaagaaaa acatgctaag aggccagatg cagatctgaa gacctatttt
      721 actgataaga aaacacatct ttacacacta atcttgaatc cagataatag ttttgaaata
      781 ctggttgacc aatctgtggt gaatagtgga aatctgctca atgacatgac tcctcctgta
      841 aatccttcac gtgaaattga ggacccagaa gaccggaagc ccgaggattg ggatgaaaga
      901 ccaaaaatcc cagatccaga agctgtcaag ccagatgact gggatgaaga tgcccctgct
      961 aagattccag atgaagaggc cacaaaaccc gaaggctggt tagatgatga gcctgagtac
     1021 gtacctgatc cagacgcaga gaaacctgag gattgggatg aagacatgga tggagaatgg
     1081 gaggctcctc agattgccaa ccctagatgt gagtcagctc ctggatgtgg tgtctggcag
     1141 cgacctgtga ttgacaaccc caattataaa ggcaaatgga agcctcctat gattgacaat
     1201 cccagttacc agggaatctg gaaacccagg aaaataccaa atccagattt ctttgaagat
     1261 ctggaacctt tcagaatgac tccttttagt gctattggtt tggagctgtg gtccatgacc
     1321 tctgacattt tttttgacaa ctttatcatt tgtgctgatc gaagaatagt tgatgattgg
     1381 gccaatgatg gatggggcct gaagaaagct gctgatgggg ctgctgagcc aggcgttgtg
     1441 gggcagatga acgaggcagc tgaagagcgc ccgtggctgt gggtagtcta tattctaact
     1501 gtagcccttc ctgtgttcct ggttatcctc ttctgctgtt ctggaaagaa acagaccagt
     1561 ggtatggagt ataagaaaac tgatgcacct caaccggatg tgaaggaaga ggaagaagag
     1621 aaggaagagg aaaaggacaa gggagatgag gaggaggaag gagaagagaa acttgaagag
     1681 aaacagaaaa gtgatgctga agaagatggt ggcactgtca gtcaagagga ggaagacaga
     1741 aaacctaaag cagaggagga tgaaattttg aacagatcac caagaaacag aaagccacga
     1801 agagaggggg gtgcgtcagg cggcagcgga tctggaccgg tcgccaccat ggtgagcaag
     1861 ggcgaggagg ataacatggc catcattaag gagttcatgc gcttcaaggt gcacatggag
     1921 gggtccgtga acggccacgt gttcgagatc gagggcgagg gcgagggccg cccctacgag
     1981 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cacctgggac
     2041 atcctgagcc ctcagttcat gtacggctcc aatgcctacg tgaagcaccc cgccgacatc
     2101 cccgactact ttaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaaattc
     2161 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     2221 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtgat gcagaagaag
     2281 accatgggct gggaggccct ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     2341 gaggtcaagc ccagagtgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     2401 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa ccgcaagctg
     2461 gacatcacca gccacaacga ggactacacc atcgtggagc agtacgagag agccgagggc
     2521 cgccactcca ccggcggcat ggacgagctg tacaagtaag gcggccgcga ct
//
