LOCUS       sequence-88316-e        1465 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Clathrin Vesicles, Excitation: 400 / 504, Emission: 
            515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1465)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-Clathrin-15
FEATURES             Location/Qualifiers
     source          1..1465
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             30..737
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(175..193)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tgacgtcaga tccgctagcg gtcgccacca tggtgagcaa gggcgaggag gataacatgg
       61 ccatcattaa ggagttcatg cgcttcaagg tgcacatgga ggggtccgtg aacggccacg
      121 tgttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
      181 tgaaggtgac caagggcggc cccctgccct tcacctggga catcctgagc cctcagttca
      241 tgtacggctc caatgcctac gtgaagcacc ccgccgacat ccccgactac tttaagctgt
      301 ccttccccga gggcttcaag tgggagcgcg tgatgaaatt cgaggacggc ggcgtggtga
      361 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
      421 gcaccaactt cccctccgac ggccccgtga tgcagaagaa gaccatgggc tgggaggccc
      481 tctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgaggtcaag cccagagtga
      541 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
      601 ccgtgcagct gcccggcgcc tacaacgtca accgcaagct ggacatcacc agccacaacg
      661 aggactacac catcgtggag cagtacgaga gagccgaggg ccgccactcc accggcggca
      721 tggacgagct gtacaagtcc ggactcagat ctcgagctca agcttcgaat tctgcagtcg
      781 acatggctga tgactttggc ttcttctcgt cgtcggagag tggtgccccg gaggcggcgg
      841 aggaggaccc ggcggccgcc ttcctggccc agcaggagag cgagattgca ggcatagaga
      901 acgacgaggg cttcggggca cctgccggca gccatgcggc ccccgcacag ccgggcccca
      961 cgagtggggc tggttctgag gacatgggga ccacagtcaa tggagatgtg tttcaggagg
     1021 ccaacggtcc tgctgatggc tacgcagcca ttgcccaggc tgacaggctg acccaggagc
     1081 ctgagagcat ccgcaagtgg cgagaggagc agaggaaacg gctgcaagag ctggatgctg
     1141 catctaaggt cacggaacag gaatggcggg agaaggccaa gaaggacctg gaggagtgga
     1201 accagcgcca gagtgaacaa gtagagaaga acaagatcaa caaccgggca tccgaggagg
     1261 ctttcgtgaa ggaatccaag gaggagaccc caggcacaga gtgggagaag gtggcccagc
     1321 tatgtgactt caaccccaag agcagcaagc agtgcaaaga tgtgtcccgc ctgcgctcgg
     1381 tgctcatgtc cctgaagcag acgccactgt cccgctaggg atccaccgga tctagataac
     1441 tgatcataat cagccatacc acatt
//
