LOCUS       sequence-88317-e        2161 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 400 / 504, Emission: 515 / 
            517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2161)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-Cx43-7
FEATURES             Location/Qualifiers
     source          1..2161
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1405..1414
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1411..2121
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1556..1574)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggactcag atctcgagct caagcttcga attcggcagc
       61 acttttcttt cattgggggg aaggcgtgag gaaagtacca aacagcagca gacttttaaa
      121 ctttaaacag acaggtctga gagcctgaac tctcattttt cctttgactt cagcctccaa
      181 ggagttccac caactttggc gcgccggctt cactttcatt aagtgaaaga gaggtgccca
      241 gacatgggtg actggagtgc cttggggaag cttctggaca aggtccaagc ctactccacc
      301 gctggaggga aggtgtggct gtcagtgctc ttcatattca gaatcctgct cctggggaca
      361 gctgttgagt cagcttgggg tgatgaacag tctgcctttc gctgtaacac tcaacaacct
      421 ggctgcgaaa acgtctgcta tgacaagtcc ttccccatct ctcacgtgcg cttctgggtc
      481 cttcagatca tattcgtgtc tgtgcccaca ctcctgtact tggcccatgt gttctatgtg
      541 atgaggaagg aagagaagct aaacaagaaa gaagaggagc tcaaagtggc ccagactgac
      601 ggggtcaacg tggagatgca cctgaagcag attgaaatca agaagttcaa gtacgggatt
      661 gaagagcacg gcaaggtgaa aatgaggggc ggcttgctga gaacctacat catcagcatc
      721 ctcttcaagt ctgtcttcga ggtggccttc ctgctcatcc agtggtacat ctatgggttc
      781 agcttgagcg cggtctacac ctgcaagaga gatccctgcc cccaccaggt agactgcttc
      841 ctctcacgtc ccacggagaa aaccatcttc atcatcttca tgctggtggt gtccttggtg
      901 tctctcgctt tgaacatcat tgagctcttc tacgtcttct tcaaaggcgt taaggatcgc
      961 gtgaagggaa gaagcgatcc ttaccacgcc accactggcc cactgagccc atcaaaagac
     1021 tgcggatctc caaaatacgc ctacttcaat ggctgctcct caccaacggc tccactctcg
     1081 cctatgtctc ctcctgggta caagctggtt actggtgaca gaaacaattc ctcgtgccgc
     1141 aattacaaca agcaagctag cgagcaaaac tgggcgaact acagcgcaga gcaaaatcgc
     1201 atggggcagg ccggaagcac catctccaac tcgcacgccc agccgttcga tttccccgac
     1261 gacaaccaga atgccaaaaa agttgctgct ggacatgaac tccagccatt agccatcgtg
     1321 gaccaacgac cttccagcag agccagcagc cgcgccagca gcaggcctcg gcctgatgac
     1381 ctggagattg cggatccacc ggtcgccacc atggtgagca agggcgagga ggataacatg
     1441 gccatcatta aggagttcat gcgcttcaag gtgcacatgg aggggtccgt gaacggccac
     1501 gtgttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     1561 ctgaaggtga ccaagggcgg ccccctgccc ttcacctggg acatcctgag ccctcagttc
     1621 atgtacggct ccaatgccta cgtgaagcac cccgccgaca tccccgacta ctttaagctg
     1681 tccttccccg agggcttcaa gtgggagcgc gtgatgaaat tcgaggacgg cggcgtggtg
     1741 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     1801 ggcaccaact tcccctccga cggccccgtg atgcagaaga agaccatggg ctgggaggcc
     1861 ctctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgaggtcaa gcccagagtg
     1921 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     1981 cccgtgcagc tgcccggcgc ctacaacgtc aaccgcaagc tggacatcac cagccacaac
     2041 gaggactaca ccatcgtgga gcagtacgag agagccgagg gccgccactc caccggcggc
     2101 atggacgagc tgtacaagta agcggccgcg actctagatc ataatcagcc ataccacatt
     2161 t
//
