LOCUS       sequence-88318-e        1681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 400 / 504, 
            Emission: 515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1681)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-EB3-7
FEATURES             Location/Qualifiers
     source          1..1681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      62..71
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      926..935
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             932..1642
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1077..1095)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac
       61 tgccaccatg gccgtcaatg tgtactccac atctgtgacc agtgaaaatc tgagtcgcca
      121 tgatatgctt gcatgggtca acgactccct gcacctcaac tataccaaga tagaacagct
      181 ttgttcaggg gcagcctact gccagttcat ggacatgctc ttccccggct gtgtgcactt
      241 gaggaaagtg aagttccagg ccaaactaga gcatgaatac atccacaact tcaaggtgct
      301 gcaagcagct ttcaagaaga tgggtgttga caaaatcatt cctgtagaga aattagtgaa
      361 aggaaaattc caagataatt ttgagtttat tcagtggttt aagaaattct ttgacgcaaa
      421 ctatgatgga aaggattaca accctctgct ggcgcggcag ggccaggacg tagcgccacc
      481 tcctaaccca ggtgatcaga tcttcaacaa atccaagaaa ctcattggca cagcagttcc
      541 acagaggacg tcccccacag gcccaaaaaa catgcagacc tctggccggc tgagcaatgt
      601 ggcccccccc tgcattctcc ggaagaatcc tccatcagcc cgaaatggcg gccatgagac
      661 tgatgcccaa attcttgaac tcaaccaaca gctggtggac ttgaagctga cagtggatgg
      721 gctggagaag gaacgtgact tctacttcag caaacttcgt gacatcgagc tcatctgcca
      781 ggagcatgaa agtgaaaaca gccctgttat ctcaggcatc attggcatcc tctatgccac
      841 agaggaagga ttcgcacccc ctgaggacga tgagattgaa gagcatcaac aagaagacca
      901 ggacgagtac ctggatccac cggtcgccac catggtgagc aagggcgagg aggataacat
      961 ggccatcatt aaggagttca tgcgcttcaa ggtgcacatg gaggggtccg tgaacggcca
     1021 cgtgttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     1081 gctgaaggtg accaagggcg gccccctgcc cttcacctgg gacatcctga gccctcagtt
     1141 catgtacggc tccaatgcct acgtgaagca ccccgccgac atccccgact actttaagct
     1201 gtccttcccc gagggcttca agtgggagcg cgtgatgaaa ttcgaggacg gcggcgtggt
     1261 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     1321 cggcaccaac ttcccctccg acggccccgt gatgcagaag aagaccatgg gctgggaggc
     1381 cctctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgaggtca agcccagagt
     1441 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     1501 gcccgtgcag ctgcccggcg cctacaacgt caaccgcaag ctggacatca ccagccacaa
     1561 cgaggactac accatcgtgg agcagtacga gagagccgag ggccgccact ccaccggcgg
     1621 catggacgag ctgtacaagt aagcggccgc gactctagat cataatcagc cataccacat
     1681 t
//
