LOCUS       sequence-88319-e        1478 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endosomes, Excitation: 400 / 504, Emission: 515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1478)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-Endo-14
FEATURES             Location/Qualifiers
     source          1..1478
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(180..198)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             788..817
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggaggataa
       61 catggccatc attaaggagt tcatgcgctt caaggtgcac atggaggggt ccgtgaacgg
      121 ccacgtgttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
      181 caagctgaag gtgaccaagg gcggccccct gcccttcacc tgggacatcc tgagccctca
      241 gttcatgtac ggctccaatg cctacgtgaa gcaccccgcc gacatccccg actactttaa
      301 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aaattcgagg acggcggcgt
      361 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
      421 gcgcggcacc aacttcccct ccgacggccc cgtgatgcag aagaagacca tgggctggga
      481 ggccctctcc gagcggatgt accccgagga cggcgccctg aagggcgagg tcaagcccag
      541 agtgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
      601 gaagcccgtg cagctgcccg gcgcctacaa cgtcaaccgc aagctggaca tcaccagcca
      661 caacgaggac tacaccatcg tggagcagta cgagagagcc gagggccgcc actccaccgg
      721 cggcatggac gagctgtaca agtccggatc agatctcgag ctcaagcttg atatcgaatt
      781 ccccatggag cagaagctga tctccgagga ggacctgaac cggaagaaac tggtggttgt
      841 tggtgatgga gcctgtggaa agacatgctt gctcatagtc ttcagcaagg acgagttccc
      901 agaggtgtat gtgcccacag tgtttgagaa ctatgtggca gatatcgagg tggatggaaa
      961 gcaggtagag ttggctttgt gggacacagc tggccaggag gactacgacc gcctgcggcc
     1021 gctctcctac ccggacaccg acgtcattct catgtgcttc tcggtggaca gcccggactc
     1081 gctggagaac atccccgaga agtgggtccc cgaggtgaag cacttctgtc ccaatgtgcc
     1141 catcatcctg gtggccaaca aaaaagacct gcgcagcgac gagcatgtcc gcacagagct
     1201 ggcccgcatg aagcaggaac ccgttcgcac ggatgacggc cgcgccatgg ccgtgcgcat
     1261 ccaagcctac gactacctcg agtgctctgc caagaccaag gaaggcgtgc gcgaggtctt
     1321 cgagacggcc acgcgcgccg cgctgcagaa gcgctacggc tcccagaacg gctgcatcaa
     1381 ctgctgcaag gtgctatgag ggccgcgccc gtcgcgcctg cccctgccgg ggatccaccg
     1441 gatctagata actgatcata atcagccata ccacattt
//
