LOCUS       sequence-88320-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 400 / 504, 
            Emission: 515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   York AG, Ghitani A, Vaziri A, Davidson MW, Shroff H
  TITLE     Confined activation and subdiffractive localization enables 
            whole-cell PALM with genetically expressed probes.
  JOURNAL   Nat Methods. 2011 Apr;8(4):327-33. doi: 10.1038/nmeth.1571. Epub 
            2011 Feb 13.
  PUBMED    21317909
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1571"; journalName: "Nat Methods"; date: "2011-04"; 
            volume: "8"; issue: "4"; pages: "327-33"
COMMENT     Sequence Label: PA-mCherry1-ER-5
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        10..61
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     12..32
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      156..165
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             162..869
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(307..325)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
       61 tggtttagtg aaccgtcaga tccgctagcg ccaccatgct gctatccgtg ccgttgctgc
      121 tcggcctcct cggcctggcc gtcgccgcac cggtcgccac catggtgagc aagggcgagg
      181 aggataacat ggccatcatt aaggagttca tgcgcttcaa ggtgcacatg gaggggtccg
      241 tgaacggcca cgtgttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
      301 agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcacctgg gacatcctga
      361 gccctcagtt catgtacggc tccaatgcct acgtgaagca ccccgccgac atccccgact
      421 actttaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaaa ttcgaggacg
      481 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
      541 tgaagctgcg cggcaccaac ttcccctccg acggccccgt gatgcagaag aagaccatgg
      601 gctgggaggc cctctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgaggtca
      661 agcccagagt gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
      721 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caaccgcaag ctggacatca
      781 ccagccacaa cgaggactac accatcgtgg agcagtacga gagagccgag ggccgccact
      841 ccaccggcgg catggacgag ctgtacaagt ccggaaagga cgagctgtaa gaattctgca
      901 gtcgacggta ccgcgggccc gggatccacc ggatctagat aactgatcat aatcagccat
      961 accacatttg tagaggtttt acttgcttta aaaa
//
