LOCUS       sequence-88322-e         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 400 / 504, Emission: 515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-Farnesyl-5
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..21
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        6..50
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     regulatory      83..92
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             89..796
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(234..252)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga
       61 accgtcagat ccgctagcgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc
      121 catcattaag gagttcatgc gcttcaaggt gcacatggag gggtccgtga acggccacgt
      181 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      241 gaaggtgacc aagggcggcc ccctgccctt cacctgggac atcctgagcc ctcagttcat
      301 gtacggctcc aatgcctacg tgaagcaccc cgccgacatc cccgactact ttaagctgtc
      361 cttccccgag ggcttcaagt gggagcgcgt gatgaaattc gaggacggcg gcgtggtgac
      421 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
      481 caccaacttc ccctccgacg gccccgtgat gcagaagaag accatgggct gggaggccct
      541 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gaggtcaagc ccagagtgaa
      601 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
      661 cgtgcagctg cccggcgcct acaacgtcaa ccgcaagctg gacatcacca gccacaacga
      721 ggactacacc atcgtggagc agtacgagag agccgagggc cgccactcca ccggcggcat
      781 ggacgagctg tacaagtccg gactcagatc taagctgaac cctcctgatg agagtggccc
      841 cggctgcatg agctgcaagt gtgtgctctc ctgagaattc tgcagtcgac ggtaccgcgg
      901 gcccgggatc caccggatct agataactga tcataatcag ccataccaca tttgtagagg
      961 ttttacttgc tttaaaaaa
//
