LOCUS       sequence-88324-e        1769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleoli, Excitation: 400 / 504, Emission: 515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1769)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-Fibrillarin-7
FEATURES             Location/Qualifiers
     source          1..1769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..735
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(173..191)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(1531..1542)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 acgtcagatc cgctagcggt cgccaccatg gtgagcaagg gcgaggagga taacatggcc
       61 atcattaagg agttcatgcg cttcaaggtg cacatggagg ggtccgtgaa cggccacgtg
      121 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
      181 aaggtgacca agggcggccc cctgcccttc acctgggaca tcctgagccc tcagttcatg
      241 tacggctcca atgcctacgt gaagcacccc gccgacatcc ccgactactt taagctgtcc
      301 ttccccgagg gcttcaagtg ggagcgcgtg atgaaattcg aggacggcgg cgtggtgacc
      361 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
      421 accaacttcc cctccgacgg ccccgtgatg cagaagaaga ccatgggctg ggaggccctc
      481 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg aggtcaagcc cagagtgaag
      541 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
      601 gtgcagctgc ccggcgccta caacgtcaac cgcaagctgg acatcaccag ccacaacgag
      661 gactacacca tcgtggagca gtacgagaga gccgagggcc gccactccac cggcggcatg
      721 gacgagctgt acaagtccgg actcagatct cgagccatga agccaggatt cagtccccgt
      781 gggggtggct ttggcggccg agggggcttt ggtgaccgtg gtggtcgtgg aggccgaggg
      841 ggctttggcg ggggccgagg ccaaggcgga ggctttagag gtcgtggacg aggaggaggt
      901 ggaggcggcg gcggcggtgg aggaggagga agaggtggtg gaggcttcca ttttggtggc
      961 aaccggggtc gtggtcgggg aggaaaaaga ggaaaccagt cggggaagaa tgtgatggtg
     1021 gagccgcatc ggcatgaggg tgtcttcatt tgtcggggaa aggaagatgc actggtcacc
     1081 aagaacctgg tccctgggga atcagtttat ggagagaaga gagtctcgat ttcggaagga
     1141 gatgacaaaa ttgagtaccg agcctggaac cccttccgct ccaagcttgc agcagcaatc
     1201 ctgggtggtg tggaccagat ccacatcaaa ccgggggcta aggttctcta cctcggggct
     1261 gcctcgggca ccacggtctc ccatgtctct gacatcgttg gtccggatgg tctagtctat
     1321 gcagtcgagt tctcccaccg ctctggccgt gacctcatta acttggccaa gaagaggacc
     1381 aacatcattc ctgtgatcga ggatgctcga cacccacaca aataccgcat gctcatcgca
     1441 atggtggatg tgatctttgc tgatgtggcc cagccagacc agacccggat tgtggccctg
     1501 aatgcccaca ccttcctgcg taatggagga cactttgtga tttccattaa ggccaactgc
     1561 attgactcca cagcctcagc cgaggccgtg tttgcctccg aagtgaaaaa gatgcaacag
     1621 gagaacatga agccgcagga gcagttgacc cttgagccat atgaaagaga ccatgccgtg
     1681 gtcgtgggag tgtacaggcc accccccaag gtgaagaact gaggatccac cggatctaga
     1741 taactgatca taatcagcca taccacatt
//
