LOCUS       sequence-88325-e        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi Complex, Excitation: 400 / 504, Emission: 515 / 
            517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-Golgi-7
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      278..287
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             284..994
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(429..447)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 cgtcagatcc gctagcatga ggcttcggga gccgctcctg agcggcagcg ccgcgatgcc
       61 aggcgcgtcc ctacagcggg cctgccgcct gctcgtggcc gtctgcgctc tgcaccttgg
      121 cgtcaccctc gtttactacc tggctggccg cgacctgagc cgcctgcccc aactggtcgg
      181 agtctccaca ccgctgcagg gcggctcgaa cagtgccgcc gccatcgggc agtcctccgg
      241 ggagctccgg accggagggg ccaaggatcc accggtcgcc accatggtga gcaagggcga
      301 ggaggataac atggccatca ttaaggagtt catgcgcttc aaggtgcaca tggaggggtc
      361 cgtgaacggc cacgtgttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
      421 ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcacct gggacatcct
      481 gagccctcag ttcatgtacg gctccaatgc ctacgtgaag caccccgccg acatccccga
      541 ctactttaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga aattcgagga
      601 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
      661 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtgatgcaga agaagaccat
      721 gggctgggag gccctctccg agcggatgta ccccgaggac ggcgccctga agggcgaggt
      781 caagcccaga gtgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
      841 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaaccgca agctggacat
      901 caccagccac aacgaggact acaccatcgt ggagcagtac gagagagccg agggccgcca
      961 ctccaccggc ggcatggacg agctgtacaa gtaagcggcc gcgactctag atcataatca
     1021 gccataccac att
//
