LOCUS       sequence-88326-e        1194 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Histones/Nucleus, Excitation: 400 / 504, Emission: 515
            / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1194)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-H2B-C-10
FEATURES             Location/Qualifiers
     source          1..1194
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             30..737
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(175..193)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             768..1145
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
ORIGIN
        1 aaacgtcaga tccgctagcg gtcgccacca tggtgagcaa gggcgaggag gataacatgg
       61 ccatcattaa ggagttcatg cgcttcaagg tgcacatgga ggggtccgtg aacggccacg
      121 tgttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
      181 tgaaggtgac caagggcggc cccctgccct tcacctggga catcctgagc cctcagttca
      241 tgtacggctc caatgcctac gtgaagcacc ccgccgacat ccccgactac tttaagctgt
      301 ccttccccga gggcttcaag tgggagcgcg tgatgaaatt cgaggacggc ggcgtggtga
      361 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
      421 gcaccaactt cccctccgac ggccccgtga tgcagaagaa gaccatgggc tgggaggccc
      481 tctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgaggtcaag cccagagtga
      541 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
      601 ccgtgcagct gcccggcgcc tacaacgtca accgcaagct ggacatcacc agccacaacg
      661 aggactacac catcgtggag cagtacgaga gagccgaggg ccgccactcc accggcggca
      721 tggacgagct gtacaagtcc ggactcagat ctggaggctc cggaggccca gagccagcga
      781 agtctgctcc cgccccgaaa aagggctcca agaaggcggt gactaaggcg cagaagaaag
      841 gcggcaagaa gcgcaagcgc agccgcaagg agagctattc catctatgtg tacaaggttc
      901 tgaagcaggt ccaccctgac accggcattt cgtccaaggc catgggcatc atgaattcgt
      961 ttgtgaacga cattttcgag cgcatcgcag gtgaggcttc ccgcctggcg cattacaaca
     1021 agcgctcgac catcacctcc agggagatcc agacggccgt gcgcctgctg ctgcctgggg
     1081 agttggccaa gcacgccgtg tccgagggta ctaaggccat caccaagtac accagcgcta
     1141 agtaaggatc caccggatct agataactga tcataatcag ccataccaca tttt
//
