LOCUS       sequence-88328-e        2113 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Note- This plasmid contains Keratin 18. Localization: Cytokeratin, 
            Excitation: 400 / 504, Emission: 515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2113)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-Keratin-17
FEATURES             Location/Qualifiers
     source          1..2113
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1357..1366
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1363..2073
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1508..1526)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gacgtcagat ccgctagcac catgagcttc accactcgct ccaccttctc caccaactac
       61 cggtccctgg gctctgtcca ggcgcccagc tacggcgccc ggccggtcag cagcgcggcc
      121 agcgtctatg caggcgctgg gggctctggt tcccggatct ccgtgtcccg ctccaccagc
      181 ttcaggggcg gcatggggtc cgggggcctg gccaccggga tagccggggg tctggcagga
      241 atgggaggca tccagaacga gaaggagacc atgcaaagcc tgaacgaccg cctggcctct
      301 tacctggaca gagtgaggag cctggagacc gagaaccgga ggctggagag caaaatccgg
      361 gagcacttgg agaagaaggg accccaggtc agagactgga gccattactt caagatcatc
      421 gaggacctga gggctcagat cttcgcaaat actgtggaca atgcccgcat cgttctgcag
      481 attgacaatg cccgtcttgc tgctgatgac tttagagtca agtatgagac agagctggcc
      541 atgcgccagt ctgtggagaa cgacatccat gggctccgca aggtcattga tgacaccaat
      601 atcacacgac tgcagctgga gacagagatc gaggctctca aggaggagct gctcttcatg
      661 aagaagaacc acgaagagga agtaaaaggc ctacaagccc agattgccag ctctgggttg
      721 accgtggagg tagatgcccc caaatctcag gacctcgcca agatcatggc agacatccgg
      781 gcccaatatg acgagctggc tcggaagaac cgagaggagc tagacaagta ctggtctcag
      841 cagattgagg agagcaccac agtgctcacc acacagtctg ctgaggttgg agctgctgag
      901 acgacgctca cagagctgag acgtacagtc cagtccttgg agatcgacct ggactccatg
      961 agaaatctga aggccagctt ggagaacagc ctgagggagg tggaggcccg ctacgcccta
     1021 cagatggagc agctcaacgg gatcctgctg caccttgagt cagagctggc acagacccgg
     1081 gcagagggac agcgccaggc ccaggagtat gaggccctgc tgaacatcaa ggtcaagctg
     1141 gaggctgaga tcgccaccta ccgccgcctg ctggaagatg gcgaggactt taatcttggt
     1201 gatgccttgg acagcagcaa ctccatgcaa accatccaaa agaccaccac ccgccggata
     1261 gtggatggca aagtggtgtc tgagaccaat gacaccaaag ttctgaggca tcgaattctg
     1321 cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag
     1381 gaggataaca tggccatcat taaggagttc atgcgcttca aggtgcacat ggaggggtcc
     1441 gtgaacggcc acgtgttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     1501 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcacctg ggacatcctg
     1561 agccctcagt tcatgtacgg ctccaatgcc tacgtgaagc accccgccga catccccgac
     1621 tactttaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa attcgaggac
     1681 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     1741 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg tgatgcagaa gaagaccatg
     1801 ggctgggagg ccctctccga gcggatgtac cccgaggacg gcgccctgaa gggcgaggtc
     1861 aagcccagag tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     1921 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaaccgcaa gctggacatc
     1981 accagccaca acgaggacta caccatcgtg gagcagtacg agagagccga gggccgccac
     2041 tccaccggcg gcatggacga gctgtacaag taaagcggcc gcgactctag atcataatca
     2101 gccataccac att
//
