LOCUS       sequence-88329-e        2798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 400 / 504, Emission: 515
            / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2798)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-LaminA-C-18
FEATURES             Location/Qualifiers
     source          1..2798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12..719
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(157..175)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 cggtcgccac catggtgagc aagggcgagg aggataacat ggccatcatt aaggagttca
       61 tgcgcttcaa ggtgcacatg gaggggtccg tgaacggcca cgtgttcgag atcgagggcg
      121 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg
      181 gccccctgcc cttcacctgg gacatcctga gccctcagtt catgtacggc tccaatgcct
      241 acgtgaagca ccccgccgac atccccgact actttaagct gtccttcccc gagggcttca
      301 agtgggagcg cgtgatgaaa ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
      361 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
      421 acggccccgt gatgcagaag aagaccatgg gctgggaggc cctctccgag cggatgtacc
      481 ccgaggacgg cgccctgaag ggcgaggtca agcccagagt gaagctgaag gacggcggcc
      541 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
      601 cctacaacgt caaccgcaag ctggacatca ccagccacaa cgaggactac accatcgtgg
      661 agcagtacga gagagccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
      721 ccggactcag atctggcagc ggtggaggca gcgcatccgg cggaagcgga agcgagaccc
      781 cgtcccagcg gcgcgccacc cgcagcgggg cgcaggccag ctccactccg ctgtcgccca
      841 cccgcatcac ccggctgcag gagaaggagg acctgcagga gctcaatgat cgcttggcgg
      901 tctacatcga ccgtgtgcgc tcgctggaaa cggagaacgc agggctgcgc cttcgcatca
      961 ccgagtctga agaggtggtc agccgcgagg tgtccggcat caaggccgcc tacgaggccg
     1021 agctcgggga tgcccgcaag acccttgact cagtagccaa ggagcgcgcc cgcctgcagc
     1081 tggagctgag caaagtgcgt gaggagttta aggagctgaa agcgcgcaat accaagaagg
     1141 agggtgacct gatagctgct caggctcggc tgaaggacct ggaggctctg ctgaactcca
     1201 aggaggccgc actgagcact gctctcagtg agaagcgcac gctggagggc gagctgcatg
     1261 atctgcgggg ccaggtggcc aagcttgagg cagccctagg tgaggccaag aagcaacttc
     1321 aggatgagat gctgcggcgg gtggatgctg agaacaggct gcagaccatg aaggaggaac
     1381 tggacttcca gaagaacatc tacagtgagg agctgcgtga gaccaagcgc cgtcatgaga
     1441 cccgactggt ggagattgac aatgggaagc agcgtgagtt tgagagccgg ctggcggatg
     1501 cgctgcagga actgcgggcc cagcatgagg accaggtgga gcagtataag aaggagctgg
     1561 agaagactta ttctgccaag ctggacaatg ccaggcagtc tgctgagagg aacagcaacc
     1621 tggtgggggc tgcccacgag gagctgcagc agtcgcgcat ccgcatcgac agcctctctg
     1681 cccagctcag ccagctccag aagcagctgg cagccaagga ggcgaagctt cgagacctgg
     1741 aggactcact ggcccgtgag cgggacacca gccggcggct gctggcggaa aaggagcggg
     1801 agatggccga gatgcgggca aggatgcagc agcagctgga cgagtaccag gagcttctgg
     1861 acatcaagct ggccctggac atggagatcc acgcctaccg caagctcttg gagggcgagg
     1921 aggagaggct acgcctgtcc cccagcccta cctcgcagcg cagccgtggc cgtgcttcct
     1981 ctcactcatc ccagacacag ggtgggggca gcgtcaccaa aaagcgcaaa ctggagtcca
     2041 ctgagagccg cagcagcttc tcacagcacg cacgcactag cgggcgcgtg gccgtggagg
     2101 aggtggatga ggagggcaag tttgtccggc tgcgcaacaa gtccaatgag gaccagtcca
     2161 tgggcaattg gcagatcaag cgccagaatg gagatgatcc cttgctgact taccggttcc
     2221 caccaaagtt caccctgaag gctgggcagg tggtgacgat ctgggctgca ggagctgggg
     2281 ccacccacag cccccctacc gacctggtgt ggaaggcaca gaacacctgg ggctgcggga
     2341 acagcctgcg tacggctctc atcaactcca ctggggaaga agtggccatg cgcaagctgg
     2401 tgcgctcagt gactgtggtt gaggacgacg aggatgagga tggagatgac ctgctccatc
     2461 accaccacgg ctcccactgc agcagctcgg gggaccccgc tgagtacaac ctgcgctcgc
     2521 gcaccgtgct gtgcgggacc tgcgggcagc ctgccgacaa ggcatctgcc agcggctcag
     2581 gagcccaggt gggcggaccc atctcctctg gctcttctgc ctccagtgtc acggtcactc
     2641 gcagctaccg cagtgtgggg ggcagtgggg gtggcagctt cggggacaat ctggtcaccc
     2701 gctcctacct cctgggcaac tccagccccc gaacccagag cccccagaac tgcagcatca
     2761 tgtaaggatc caccggatct agataactga tcataatc
//
