LOCUS       sequence-88330-e        2556 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 400 / 504, Emission: 515
            / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   York AG, Ghitani A, Vaziri A, Davidson MW, Shroff H
  TITLE     Confined activation and subdiffractive localization enables 
            whole-cell PALM with genetically expressed probes.
  JOURNAL   Nat Methods. 2011 Apr;8(4):327-33. doi: 10.1038/nmeth.1571. Epub 
            2011 Feb 13.
  PUBMED    21317909
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1571"; journalName: "Nat Methods"; date: "2011-04"; 
            volume: "8"; issue: "4"; pages: "327-33"
COMMENT     Sequence Label: PA-mCherry1-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..2556
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..736
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(174..192)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aacgtcagat ccgctagcgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc
       61 catcattaag gagttcatgc gcttcaaggt gcacatggag gggtccgtga acggccacgt
      121 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      181 gaaggtgacc aagggcggcc ccctgccctt cacctgggac atcctgagcc ctcagttcat
      241 gtacggctcc aatgcctacg tgaagcaccc cgccgacatc cccgactact ttaagctgtc
      301 cttccccgag ggcttcaagt gggagcgcgt gatgaaattc gaggacggcg gcgtggtgac
      361 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
      421 caccaacttc ccctccgacg gccccgtgat gcagaagaag accatgggct gggaggccct
      481 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gaggtcaagc ccagagtgaa
      541 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
      601 cgtgcagctg cccggcgcct acaacgtcaa ccgcaagctg gacatcacca gccacaacga
      661 ggactacacc atcgtggagc agtacgagag agccgagggc cgccactcca ccggcggcat
      721 ggacgagctg tacaagtccg gactcagatc tcgagctcaa gcttcgatgg cgactgcgac
      781 ccccgtgccg ccgcggatgg gcagccgcgc tggcggcccc accacgccgc tgagccccac
      841 gcgcctgtcg cggctccagg agaaggagga gctgcgcgag ctcaatgacc ggctggcggt
      901 gtacatcgac aaggtgcgca gcctggagac ggagaacagc gcgctgcagc tgcaggtgac
      961 ggagcgcgag gaggtgcgcg gccgtgagct caccggcctc aaggcgctct acgagaccga
     1021 gctggccgac gcgcgacgcg cgctcgacga cacggcccgc gagcgcgcca agctgcagat
     1081 cgagctgggc aagtgcaagg cggaacacga ccagctgctc ctcaactatg ctaagaagga
     1141 atctgatctt aatggcgccc agatcaagct tcgagaatat gaagcagcac tcaattcgaa
     1201 agatgcagct cttgctactg cacttggtga caaaaaaagt ttagagggag atttggagga
     1261 tctgaaggat cagattgccc agttggaagc ctccttagct gcagccaaaa aacagttagc
     1321 agatgaaact ttacttaaag tagatttgga gaatcgttgt cagagcctta ctgaggactt
     1381 ggagtttcgc aaaagcatgt atgaagagga gattaacgag accagaagga agcatgaaac
     1441 gcgcttggta gaggtggatt ctgggcgtca aattgagtat gagtacaagc tggcgcaagc
     1501 ccttcatgag atgagagagc aacatgatgc ccaagtgagg ctgtataagg aggagctgga
     1561 gcagacttac catgccaaac ttgagaatgc cagactgtca tcagagatga atacttctac
     1621 tgtcaacagt gccagggaag aactgatgga aagccgcatg agaattgaga gcctttcatc
     1681 ccagctttct aatctacaga aagagtctag agcatgtttg gaaaggattc aagaattaga
     1741 ggacttgctt gctaaagaaa aagacaactc tcgtcgcatg ctgacagaca aagagagaga
     1801 gatggcggaa ataagggatc aaatgcagca acagctgaat gactatgaac agcttcttga
     1861 tgtaaagtta gccctggaca tggaaatcag tgcttacagg aaactcttag aaggcgaaga
     1921 agagaggttg aagctgtctc caagcccttc ttcccgtgtg acagtatccc gagcatcctc
     1981 aagtcgtagt gtacgtacaa ctagaggaaa gcggaagagg gttgatgtgg aagaatcaga
     2041 ggcgagtagt agtgttagca tctctcattc cgcctcagcc actggaaatg tttgcatcga
     2101 agaaattgat gttgatggga aatttatccg cttgaagaac acttctgaac aggatcaacc
     2161 aatgggaggc tgggagatga tcagaaaaat tggagacaca tcagtcagtt ataaatatac
     2221 ctcaagatat gtgctgaagg caggccagac tgttacaatt tgggctgcaa acgctggtgt
     2281 cacagccagc cccccaactg acctcatctg gaagaaccag aactcgtggg gcactggcga
     2341 agatgtgaag gttatattga aaaattctca gggagaggag gttgctcaaa gaagtacagt
     2401 ctttaaaaca accatacctg aagaagagga ggaggaggaa gaagcagctg gagtggttgt
     2461 tgaggaagaa cttttccacc agcagggaac cccaagagca tccaatagaa gctgtgcaat
     2521 tatgtaggga tccaccggat ctagataact gatcat
//
