LOCUS       sequence-88333-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 400 / 504, Emission: 515 / 
            517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   York AG, Ghitani A, Vaziri A, Davidson MW, Shroff H
  TITLE     Confined activation and subdiffractive localization enables 
            whole-cell PALM with genetically expressed probes.
  JOURNAL   Nat Methods. 2011 Apr;8(4):327-33. doi: 10.1038/nmeth.1571. Epub 
            2011 Feb 13.
  PUBMED    21317909
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1571"; journalName: "Nat Methods"; date: "2011-04"; 
            volume: "8"; issue: "4"; pages: "327-33"
COMMENT     Sequence Label: PA-mCherry1-Mito-7
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        39..90
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     41..61
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             119..193
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      221..230
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             227..937
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(372..390)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
       61 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcat
      121 gtccgtcctg acgccgctgc tgctgcgggg cttgacaggc tcggcccggc ggctcccagt
      181 gccgcgcgcc aagatccatt cgttggggga tccaccggtc gccaccatgg tgagcaaggg
      241 cgaggaggat aacatggcca tcattaagga gttcatgcgc ttcaaggtgc acatggaggg
      301 gtccgtgaac ggccacgtgt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
      361 cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttca cctgggacat
      421 cctgagccct cagttcatgt acggctccaa tgcctacgtg aagcaccccg ccgacatccc
      481 cgactacttt aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaaattcga
      541 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
      601 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtgatgc agaagaagac
      661 catgggctgg gaggccctct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
      721 ggtcaagccc agagtgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
      781 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaacc gcaagctgga
      841 catcaccagc cacaacgagg actacaccat cgtggagcag tacgagagag ccgagggccg
      901 ccactccacc ggcggcatgg acgagctgta caagtaagcg gccgcgactc tagatcataa
      961 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     1021 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgt
//
