LOCUS       sequence-88334-e        2324 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 400 / 504, Emission: 515 / 
            517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2324)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-Myotilin-C-14
FEATURES             Location/Qualifiers
     source          1..2324
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..741
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(179..197)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggaggataac
       61 atggccatca ttaaggagtt catgcgcttc aaggtgcaca tggaggggtc cgtgaacggc
      121 cacgtgttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      181 aagctgaagg tgaccaaggg cggccccctg cccttcacct gggacatcct gagccctcag
      241 ttcatgtacg gctccaatgc ctacgtgaag caccccgccg acatccccga ctactttaag
      301 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga aattcgagga cggcggcgtg
      361 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
      421 cgcggcacca acttcccctc cgacggcccc gtgatgcaga agaagaccat gggctgggag
      481 gccctctccg agcggatgta ccccgaggac ggcgccctga agggcgaggt caagcccaga
      541 gtgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
      601 aagcccgtgc agctgcccgg cgcctacaac gtcaaccgca agctggacat caccagccac
      661 aacgaggact acaccatcgt ggagcagtac gagagagccg agggccgcca ctccaccggc
      721 ggcatggacg agctgtacaa gtccggaagt gctgggagcg caagcggaag cggaggctcc
      781 ggttttaact acgaacgtcc aaaacacttc atccagtccc aaaacccatg tggctccaga
      841 ttgcagcctc ctggaccaga aacctccagc ttctctagcc agaccaaaca gtcttccatt
      901 atcatccagc cccgccagtg tacagagcaa agattttctg cctcctcaac actgagctct
      961 cacatcacca tgtcctcctc tgctttccct gcttctcccc agcagcatgc tggctccaac
     1021 ccaggccaaa gggttacaac cacctataac cagtccccag ccagcttcct cagctccata
     1081 ttaccatcac agcctgatta caatagcagt aaaatccctt ccgctatgga ttccaactat
     1141 caacagtcct cagctggcca acctataaat gcaaagccat cccaaactgc aaatgctaag
     1201 cccataccaa gaactcctga tcatgaaata caaggatcaa aagaagcttt gattcaagat
     1261 ttggaaagaa agctgaaatg caaggacacc cttcttcata atggaaatca acgtctaaca
     1321 tatgaagaga agatggctcg cagattgcta ggaccacaga atgcagctgc tgtgtttcaa
     1381 gctcaggatg acagtggtgc acaagactcg cagcaacaca actcagaaca tgcgcgactg
     1441 caagttccta catcacaagt aagaagtaga tcaacctcaa ggggagatgt gaatgatcag
     1501 gatgcaatcc aggagaaatt ttacccacca cgtttcattc aagtgccaga gaacatgtcg
     1561 attgatgaag gaagattctg cagaatggac ttcaaagtga gtggactgcc agctcctgat
     1621 gtgtcatggt atctaaatgg aagaacagtt caatcagatg atttgcacaa aatgatagtg
     1681 tctgagaagg gtcttcattc actcatcttt gaagtagtca gagcttcaga tgcaggggct
     1741 tatgcatgtg ttgccaagaa tagagcagga gaagccacct tcactgtgca gctggatgtc
     1801 cttgcaaaag aacataaaag agcaccaatg tttatctaca aaccacagag caaaaaagtt
     1861 ttagagggag attcagtgaa actagaatgc cagatctcgg ctatacctcc accaaagctt
     1921 ttctggaaaa gaaataatga aatggtacaa ttcaacactg accgaataag cttatatcaa
     1981 gataacactg gaagagttac tttactgata aaagatgtaa acaagaaaga tgctgggtgg
     2041 tatactgtgt cagcagttaa tgaagctgga gtgactacat gtaacacaag attagacgtt
     2101 acggcacgtc caaaccaaac tcttccagct cctaagcagt tacgggttcg accaacattc
     2161 agcaaatatt tagcacttaa tgggaaaggt ttgaatgtaa aacaagcttt taacccagaa
     2221 ggagaatttc agcgtttggc agctcaatct ggactctatg aaagtgaaga actttaagga
     2281 tccaccggat ctagataact gatcataatc agccatacca catt
//
