LOCUS       sequence-88338-e        2695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Fimbrin/Cytoskeleton, Excitation: 400 / 504, Emission:
            515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2695)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-Plastin-C-10
FEATURES             Location/Qualifiers
     source          1..2695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..741
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(179..197)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     RBS             2103..2111
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggaggataac
       61 atggccatca ttaaggagtt catgcgcttc aaggtgcaca tggaggggtc cgtgaacggc
      121 cacgtgttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      181 aagctgaagg tgaccaaggg cggccccctg cccttcacct gggacatcct gagccctcag
      241 ttcatgtacg gctccaatgc ctacgtgaag caccccgccg acatccccga ctactttaag
      301 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga aattcgagga cggcggcgtg
      361 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
      421 cgcggcacca acttcccctc cgacggcccc gtgatgcaga agaagaccat gggctgggag
      481 gccctctccg agcggatgta ccccgaggac ggcgccctga agggcgaggt caagcccaga
      541 gtgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
      601 aagcccgtgc agctgcccgg cgcctacaac gtcaaccgca agctggacat caccagccac
      661 aacgaggact acaccatcgt ggagcagtac gagagagccg agggccgcca ctccaccggc
      721 ggcatggacg agctgtacaa gtccggaagc ggctccggga gcggaagcgg ggaaaacagt
      781 actactacca tttctcggga ggagcttgaa gaactacaag aggcatttaa taaaatagat
      841 attgacaata gtgggtatgt cagtgactat gaacttcaag acctgtttaa ggaagcaagc
      901 cttcctctgc ctggctacaa ggtgcgcgag attgtggaga aaattctatc agttgctgac
      961 agcaacaaag atggcaaaat cagttttgaa gagtttgtgt cactaatgca agaattaaaa
     1021 agcaaagata tcagcaaaac attccgaaaa ataattaaca agagggaagg gattactgct
     1081 attggaggaa cttcaactat ttccagtgag ggcacacagc attcttattc agaggaagaa
     1141 aaagtggctt ttgttaactg gataaacaaa gccctggaga atgaccctga ctgtaagcat
     1201 cttataccca tgaatcccaa tgatgatagt cttttcaagt cacttgcaga tggcatcctt
     1261 ctttgcaaaa tgatcaactt atctgaacca gatacaattg atgaaagagc catcaataag
     1321 aaaaagctca cgccattcac tatttctgaa aatttaaacc tagctctgaa ttctgcctca
     1381 gccattggtt gtacagtggt caacattggt gcatcagatc tcaaagaagg aaaacctcac
     1441 ttggtcttgg gacttctctg gcagatcatc aaagttggcc tttttgctga tattgagatt
     1501 tccaggaatg aagctctgat tgcattgtta aatgaaggtg aggaactaga ggagctgatg
     1561 aagctttctc ccgaggaatt actgctgcga tgggtgaact accatctgac caatgcagga
     1621 tggcatacca tcagcaactt cagccaagac attaaggact cgagagccta ttttcatctg
     1681 cttaatcaga ttgcccctaa aggtggggaa gatggacctg ccattgccat tgacctttca
     1741 ggaattaatg agacaaatga cctgaagcgt gctggactca tgcttcaaga agcagataaa
     1801 ctgggctgca aacagtttgt tactcctgca gatgtggttt caggcaatcc taaacttaat
     1861 ttagcttttg tagctaattt gtttaacaca tacccgtgcc tgcacaagcc gaataataat
     1921 gacatcgata tgaatttact ggaaggagag agcaaggaag agagaacatt tcggaactgg
     1981 atgaattcct tgggagtcaa cccatacatt aatcatttgt acagtgacct tgcagatgct
     2041 ttagtgatct ttcagctcta tgagatgatc cgagtgccag tcaactggag ccatgtcaac
     2101 aaacctcctt atcctgccct tggagggaac atgaagaaga ttgaaaactg taactatgca
     2161 gtggaacttg ggaagaacaa ggccaaattc tccttggttg gcattgctgg gcaggaccta
     2221 aatgaaggga attcaacact taccctggca ttggtatggc agctgatgag aaggtacaca
     2281 ttgaatgtgt tatcggatct tggagagggt gaaaaagtaa atgatgaaat tataattaaa
     2341 tgggtcaatc agactcttaa aagtgcaaac aaaaagactt ctatttccag cttcaaggat
     2401 aaatctataa gcacaagttt acctgtccta gatttaatag atgccattgc accaaatgca
     2461 gttcgtcaag aaatgatcag gagagaaaac ttatctgatg aggacaagct gaacaatgct
     2521 aaatacgcca tttcagttgc tcgaaagatc ggtgcccgga tatatgcatt acctgatgac
     2581 ctcgtagaag tgaaaccaaa gatggttatg acggtgtttg catgcttaat gggaaaagga
     2641 ctgaacagaa taaaataagg atccaccgga tctagataac tgatcataat cagcc
//
