LOCUS       sequence-88341-e         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi TGN, Excitation: 400 / 504, Emission: 515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-SiT-N-15
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      217..226
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             223..933
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(368..386)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagctcgcca ccatgattca caccaacctg
       61 aagaaaaagt tcagctgctg cgtcctggtc tttcttctgt ttgcagtcat ctgtgtgtgg
      121 aaggaaaaga agaaagggag ttactatgat tcctttaaat tgcaaaccaa ggaattcctg
      181 cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag
      241 gaggataaca tggccatcat taaggagttc atgcgcttca aggtgcacat ggaggggtcc
      301 gtgaacggcc acgtgttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
      361 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcacctg ggacatcctg
      421 agccctcagt tcatgtacgg ctccaatgcc tacgtgaagc accccgccga catccccgac
      481 tactttaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa attcgaggac
      541 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
      601 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg tgatgcagaa gaagaccatg
      661 ggctgggagg ccctctccga gcggatgtac cccgaggacg gcgccctgaa gggcgaggtc
      721 aagcccagag tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
      781 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaaccgcaa gctggacatc
      841 accagccaca acgaggacta caccatcgtg gagcagtacg agagagccga gggccgccac
      901 tccaccggcg gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag
      961 ccat
//
